Method Development and Total Diet Research Center, U.S. Food and Drug Administration, 11630 W. 80th Street, Lenexa, KS 66214, USA.
J Pharm Biomed Anal. 2012 Nov;70:40-6. doi: 10.1016/j.jpba.2012.05.017. Epub 2012 May 23.
A flow injection tandem mass spectrometry method (FI-MS/MS) has been developed to detect enzyme phosphodiesterase type 5 inhibitors, including tadalafil, sildenafil, and vardenafil. Multiple reaction monitoring (MRM) was used to detect the drugs and product ion ratios were used for identification. FI-MS/MS was used for semi-quantification and liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for further confirmation and quantification. One of 13 samples has been found to be adulterated with prescription levels of tadalafil and also low level of sildenafil. The method can be used for screening large numbers of herbal products for adulteration since it takes less than 1 min without chromatographic separation on a column.
建立了一种用于检测酶磷酸二酯酶 5 抑制剂（包括他达拉非、西地那非和伐地那非）的流动注射串联质谱法（FI-MS/MS）。多反应监测（MRM）用于检测药物，并用产物离子比进行鉴定。FI-MS/MS 用于半定量，液相色谱串联质谱（LC-MS/MS）用于进一步确证和定量。在 13 个样品中发现有一个掺有处方剂量的他达拉非和低水平的西地那非。由于该方法不需要柱色谱分离，整个过程不到 1 分钟，因此可用于筛选大量掺假的中草药产品。
