小鼠乳腺肿瘤病毒DNA在大鼠肝癌细胞中的整合与转录。
Integration and transcription of mouse mammary tumor virus DNA in rat hepatoma cells.
作者信息
Ringold G M, Shank P R, Varmus H E, Ring J, Yamamoto K R
出版信息
Proc Natl Acad Sci U S A. 1979 Feb;76(2):665-9. doi: 10.1073/pnas.76.2.665.
Rat hepatoma cells infected with mouse mammary tumor virus (MMTV) acquire viral DNA that becomes covalently linked to the cell DNA. Using restriction endonucleases and the DNA transfer procedure of Southern [Southern , E.M. (1975) J. Mol. Biol. 98, 503--517], we have studied the sites in cellular DNA into which MMTV DNA inserts. These experiments indicate that: (i) there are many sites in cell DNA into which MMTV DNA integrates; (ii) the junctions between viral and cellular DNA occur within a limited portion of the viral genome, (iii) clones that contain MMTV DNA do not necessarily produce viral RNA; and (iv) the extent of transcription and glucocorticoid responsiveness of MMTV proviruses may be dependent on the site(s) in cell DNA in which the viral DNA resides.
感染了小鼠乳腺肿瘤病毒（MMTV）的大鼠肝癌细胞会获得与细胞DNA共价连接的病毒DNA。利用限制性内切酶和Southern [Southern, E.M. (1975) J. Mol. Biol. 98, 503 - 517] 的DNA转移程序，我们研究了MMTV DNA插入细胞DNA的位点。这些实验表明：（i）细胞DNA中有许多MMTV DNA整合的位点；（ii）病毒DNA与细胞DNA之间的连接发生在病毒基因组的有限部分内；（iii）含有MMTV DNA的克隆不一定产生病毒RNA；（iv）MMTV前病毒的转录程度和糖皮质激素反应性可能取决于病毒DNA所在的细胞DNA位点。
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