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克隆的小鼠乳腺肿瘤病毒DNA在转染的小鼠细胞中具有生物活性，其表达受糖皮质激素刺激。

Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones.

作者信息

Buetti E, Diggelmann H

出版信息

Cell. 1981 Feb;23(2):335-45. doi: 10.1016/0092-8674(81)90129-x.

DOI:10.1016/0092-8674(81)90129-x
PMID:6258799
Abstract

We have cloned circular unintegrated mouse mammary tumor virus (MMTV) DNA from infected rat hepatoma cells in bacteriophage lambda. Seven independent clones containing MMTV DNA of homogeneous length of 9 kb (five) or 10 kb (two) were identified. The five 9 kb clones had identical restriction maps consistent with that of 9 kb unintegrated DNA; the other two were aberrant. MMTV DNA inserts were purified, ligated and used for cotransfection of Ltk- cells together with a plasmid containing the thymidine kinase gene of herpes simplex virus. All Tk+ cell clones acquired new MMTV sequences and those transfected with the 9 kb MMTV DNA synthesized normal viral RNA and proteins. Viral gene expression was increased by the addition of dexamethasone.

摘要

我们从感染的大鼠肝癌细胞中，在噬菌体λ载体中克隆出了环状未整合的小鼠乳腺肿瘤病毒（MMTV）DNA。鉴定出了七个独立的克隆，其中五个包含长度均一为9 kb的MMTV DNA，另外两个为10 kb。这五个9 kb的克隆具有与9 kb未整合DNA一致的相同限制性图谱；另外两个则异常。纯化、连接MMTV DNA插入片段，并将其与含有单纯疱疹病毒胸苷激酶基因的质粒一起用于共转染Ltk-细胞。所有Tk+细胞克隆都获得了新的MMTV序列，用9 kb MMTV DNA转染的细胞克隆合成了正常的病毒RNA和蛋白质。通过添加地塞米松可增加病毒基因表达。

相似文献

1
Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones.克隆的小鼠乳腺肿瘤病毒DNA在转染的小鼠细胞中具有生物活性，其表达受糖皮质激素刺激。
Cell. 1981 Feb;23(2):335-45. doi: 10.1016/0092-8674(81)90129-x.
2
Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.小鼠乳腺肿瘤病毒内源性前病毒基因在分子克隆并转入培养细胞后受激素应答的表达
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038-42. doi: 10.1073/pnas.78.4.2038.
3
Cloned mouse mammary tumor virus DNA exhibits glucocorticoid-dependent expression in simian virus 40-transformed mink cells.克隆的小鼠乳腺肿瘤病毒DNA在猿猴病毒40转化的貂细胞中表现出糖皮质激素依赖性表达。
J Virol. 1983 Jan;45(1):148-54. doi: 10.1128/JVI.45.1.148-154.1983.
4
Cloned endogenous mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones.克隆的内源性小鼠乳腺肿瘤病毒DNA在转染的小鼠细胞中具有生物活性，其表达受糖皮质激素刺激。
Virology. 1982 Oct 30;122(2):332-41. doi: 10.1016/0042-6822(82)90233-1.
5
Glucocorticoid regulation of the Ha-MuSV p21 gene conferred by sequences from mouse mammary tumor virus.由小鼠乳腺肿瘤病毒序列赋予的糖皮质激素对Ha-MuSV p21基因的调控
Cell. 1981 Dec;27(2 Pt 1):245-55. doi: 10.1016/0092-8674(81)90408-6.
6
Structure, stability, methylation, expression and glucocorticoid induction of endogenous and transfected proviral genes of mouse mammary tumor virus in mouse fibroblasts.小鼠成纤维细胞中小鼠乳腺肿瘤病毒内源性和转染前病毒基因的结构、稳定性、甲基化、表达及糖皮质激素诱导作用
Gene. 1981 Dec;15(4):307-17. doi: 10.1016/0378-1119(81)90174-8.
7
Dexamethasone increases the number of RNA polymerase II molecules transcribing integrated mouse mammary tumor virus DNA and flanking mouse sequences.地塞米松增加了转录整合的小鼠乳腺肿瘤病毒DNA及侧翼小鼠序列的RNA聚合酶II分子的数量。
Mol Cell Biol. 1984 Jun;4(6):1057-62. doi: 10.1128/mcb.4.6.1057-1062.1984.
8
Altered effects of glucocorticoids on the trafficking and processing of mouse mammary tumor virus glycoproteins constitutively expressed in rat hepatoma cells in the absence of nonglycosylated viral components.在不存在非糖基化病毒成分的情况下，糖皮质激素对在大鼠肝癌细胞中组成性表达的小鼠乳腺肿瘤病毒糖蛋白的运输和加工的影响发生改变。
Mol Endocrinol. 1991 Nov;5(11):1696-706. doi: 10.1210/mend-5-11-1696.
9
Mouse mammary tumor virus genes: regulation of expression by glucocorticoids and structural analysis with restriction endonucleases.
J Toxicol Environ Health. 1978 Mar-May;4(2-3):457-70. doi: 10.1080/15287397809529671.
10
Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome.许多由小鼠乳腺肿瘤病毒诱发的肿瘤都含有一个整合在宿主基因组同一区域的前病毒。
Cell. 1982 Nov;31(1):99-109. doi: 10.1016/0092-8674(82)90409-3.

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Viruses. 2022 Aug 31;14(9):1941. doi: 10.3390/v14091941.
2
Glucocorticoid regulation of mouse mammary tumor virus: identification of a short essential DNA region.糖皮质激素对小鼠乳腺肿瘤病毒的调控：一个短的必需DNA区域的鉴定
EMBO J. 1983;2(8):1423-9. doi: 10.1002/j.1460-2075.1983.tb01601.x.
3
Reverse transcriptase of mouse mammary tumour virus: expression in bacteria, purification and biochemical characterization.
小鼠乳腺肿瘤病毒逆转录酶：在细菌中的表达、纯化及生化特性分析
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Differential effects of nerve growth factor and dexamethasone on herpes simplex virus type 1 oriL- and oriS-dependent DNA replication in PC12 cells.神经生长因子和地塞米松对PC12细胞中单纯疱疹病毒1型oriL和oriS依赖性DNA复制的不同作用。
J Virol. 1997 May;71(5):3580-7. doi: 10.1128/JVI.71.5.3580-3587.1997.
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Chromatin structure of the MMTV promoter and its changes during hormonal induction.MMTV启动子的染色质结构及其在激素诱导过程中的变化。
Cell Mol Neurobiol. 1996 Apr;16(2):85-101. doi: 10.1007/BF02088169.
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Stably integrated mouse mammary tumor virus long terminal repeat DNA requires the octamer motifs for basal promoter activity.稳定整合的小鼠乳腺肿瘤病毒长末端重复序列DNA需要八聚体基序来实现基础启动子活性。
Mol Cell Biol. 1994 Feb;14(2):1191-203. doi: 10.1128/mcb.14.2.1191-1203.1994.
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Tissue-specific and ubiquitous factors binding next to the glucocorticoid receptor modulate transcription from the mouse mammary tumor virus promoter.结合在糖皮质激素受体旁的组织特异性和普遍存在的因子调节来自小鼠乳腺肿瘤病毒启动子的转录。
J Virol. 1995 Jun;69(6):3759-70. doi: 10.1128/JVI.69.6.3759-3770.1995.
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Induction of human beta-interferon synthesis with poly(rI . rC) in mouse cells transfected with cloned cDNA plasmids.用聚（rI·rC）在转染了克隆cDNA质粒的小鼠细胞中诱导人β-干扰素合成。
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4337-41. doi: 10.1073/pnas.79.14.4337.
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Introduction, stable integration, and controlled expression of a chimeric adenovirus gene whose product is toxic to the recipient human cell.嵌合腺病毒基因的导入、稳定整合及可控表达，该基因的产物对受体人类细胞有毒性。
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Mol Cell Biol. 1984 Jun;4(6):1057-62. doi: 10.1128/mcb.4.6.1057-1062.1984.