与禽肿瘤病毒相关的脱氧核糖核酸聚合酶：脱氧核糖核酸产物的二级结构
Deoxyribonucleic acid polymerase associated with avian tumor viruses: secondary structure of the deoxyribonucleic acid product.
作者信息
Fanshier L, Garapin A C, McDonnell J, Faras A, Levinson W, Bishop J M
出版信息
J Virol. 1971 Jan;7(1):77-86. doi: 10.1128/JVI.7.1.77-86.1971.
Abstract
The products of the deoxyribonucleic acid (DNA) polymerase associated with Rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses with equilibrium centrifugation and stepwise elution from hydroxyapatite. The initial enzymatic product consists of nascent DNA chains which are hydrogen-bonded to 70S viral ribonucleic acid (RNA), whereas the final enzymatic product is double-stranded DNA. Appreciable amounts of free single-stranded DNA were not detected at any point during the course of the enzymatic reaction, but the data in this regard are not decisive. The time course of synthesis of DNA:RNA hybrids and double-stranded DNA has been analyzed. It is concluded that the synthesis of double-stranded DNA is a sequel to and is probably dependent upon the synthesis of DNA:RNA hybrid.
摘要
通过平衡离心和从羟基磷灰石上逐步洗脱进行相关分析，对与劳氏肉瘤病毒和禽成髓细胞瘤病毒相关的脱氧核糖核酸（DNA）聚合酶的产物进行了表征。最初的酶促产物由与70S病毒核糖核酸（RNA）氢键结合的新生DNA链组成，而最终的酶促产物是双链DNA。在酶促反应过程中的任何时候都未检测到大量游离单链DNA，但这方面的数据并不具有决定性。已对DNA:RNA杂种和双链DNA的合成时间进程进行了分析。得出的结论是，双链DNA的合成是DNA:RNA杂种合成的后续过程，并且可能依赖于DNA:RNA杂种的合成。
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