从弧菌 CN41 中克隆、表达和表征一种新型β-琼脂酶。
Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41.
机构信息
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
出版信息
Appl Environ Microbiol. 2011 Oct;77(19):7077-9. doi: 10.1128/AEM.05364-11. Epub 2011 Aug 5.
Abstract
A new agarase, AgaA(CN41), cloned from Vibrio sp. strain CN41, consists of 990 amino acids, with only 49% amino acid sequence identity with known β-agarases. AgaA(CN41) belongs to the GH50 (glycoside hydrolase 50) family but yields neoagarotetraose as the end product. AgaA(CN41) was expressed and characterized.
摘要
一种新型琼脂酶 AgaA(CN41)，从弧菌属菌株 CN41 中克隆得到，由 990 个氨基酸组成，与已知的β-琼脂酶只有 49%的氨基酸序列同一性。AgaA(CN41)属于 GH50（糖苷水解酶 50）家族，但产物为新琼四糖。AgaA(CN41)被表达和表征。
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