对来自弧菌属菌株JT0107的一种新型β-琼脂酶的agaB基因进行序列分析。
Sequence analysis of the agaB gene encoding a new beta-agarase from Vibrio sp. strain JT0107.
作者信息
Sugano Y, Matsumoto T, Noma M
机构信息
Seawater Science Research Laboratory, Japan Tobacco Inc., Kanagawa.
出版信息
Biochim Biophys Acta. 1994 May 17;1218(1):105-8. doi: 10.1016/0167-4781(94)90109-0.
An agarase gene (agaB) was cloned from genomic DNA of Vibrio sp. strain JT0107. Analysis of the 3200 nucleotide sequence just before the agarase 0107 gene (agaA) which existed in genomic DNA of Vibrio sp. strain JT0107 revealed a putative single open reading frame coding for 955 amino acids. Comparison of the deduced amino acid sequence of AgaB to that of agarase 0107 revealed the existence of partially highly homologous regions. A part of this gene was expressed in Escherichia coli to yield a protein with agarase activity. This is the first report of evidence by genetic analysis that at least two different kinds of agarases exist in strain JT0107.
从弧菌属菌株JT0107的基因组DNA中克隆出一个琼脂酶基因（agaB）。对存在于弧菌属菌株JT0107基因组DNA中的琼脂酶0107基因（agaA）之前的3200个核苷酸序列进行分析，发现一个推定的单一开放阅读框，编码955个氨基酸。将推导的AgaB氨基酸序列与琼脂酶0107的氨基酸序列进行比较，发现存在部分高度同源区域。该基因的一部分在大肠杆菌中表达，产生具有琼脂酶活性的蛋白质。这是通过遗传分析首次证明菌株JT0107中至少存在两种不同类型琼脂酶的证据报告。
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