Miyazono Ken-ichi, Tabei Nobumitsu, Marushima Kazuya, Ohnishi Yasuo, Horinouchi Sueharu, Tanokura Masaru
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):914-6. doi: 10.1107/S1744309111022275. Epub 2011 Jul 20.
Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293 K. A crystal of SgGlkA in complex with glucose was obtained using a reservoir solution consisting of 0.9 M sodium/potassium tartrate, 0.2 M NaCl and 0.1 M imidazole pH 8.1 and diffracted X-rays to 1.84 Å resolution. The crystal of SgGlkA in complex with glucose belonged to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 109.19, c = 141.18 Å. The crystal contained one molecule in the asymmetric unit.
葡萄糖激酶利用ATP催化葡萄糖磷酸化生成6-磷酸葡萄糖。SgGlkA是来自灰色链霉菌的一种细菌III型葡萄糖激酶，似乎在该生物体的碳分解代谢物阻遏中发挥调节作用。SgGlkA在大肠杆菌中表达，经纯化后于293 K使用坐滴气相扩散法进行结晶。使用由0.9 M酒石酸钠/钾、0.2 M NaCl和0.1 M咪唑（pH 8.1）组成的储液获得了与葡萄糖结合的SgGlkA晶体，该晶体的X射线衍射分辨率为1.84 Å。与葡萄糖结合的SgGlkA晶体属于空间群P6(2)22或P6(4)22，晶胞参数a = b = 109.19，c = 141.18 Å。该晶体在不对称单元中包含一个分子。
