嗜热栖热菌一种假定的葡萄糖激酶/己糖激酶的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of a putative glucokinase/hexokinase from Thermus thermophilus.
作者信息
Nakamura Tsutomu, Kashima Yasuhiro, Mine Shouhei, Oku Takashi, Uegaki Koichi
机构信息
National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1559-62. doi: 10.1107/S1744309111041145. Epub 2011 Nov 25.
Abstract
Glucokinase/hexokinase catalyzes the phosphorylation of glucose to glucose 6-phosphate, which is the first step of glycolysis. The open reading frame TTHA0299 of the extreme thermophile Thermus thermophilus encodes a putative glucokinase/hexokinase which contains the consensus sequence for proteins from the repressors, open reading frames and sugar kinases family. In this study, the glucokinase/hexokinase from T. thermophilus was purified and crystallized using polyethylene glycol 8000 as a precipitant. Diffraction data were collected and processed to 2.02 Å resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 70.93, b = 138.14, c = 75.16 Å, β = 95.41°.
摘要
葡萄糖激酶/己糖激酶催化葡萄糖磷酸化生成6-磷酸葡萄糖，这是糖酵解的第一步。嗜热栖热菌（Thermus thermophilus）的开放阅读框TTHA0299编码一种假定的葡萄糖激酶/己糖激酶，它包含来自阻遏物、开放阅读框和糖激酶家族蛋白质的共有序列。在本研究中，使用聚乙二醇8000作为沉淀剂对嗜热栖热菌的葡萄糖激酶/己糖激酶进行了纯化和结晶。收集了衍射数据并处理至2.02 Å分辨率。晶体属于空间群P2(1)，晶胞参数为a = 70.93，b = 138.14，c = 75.16 Å，β = 95.41°。
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