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本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
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Molecular replacement with MOLREP.使用MOLREP进行分子置换。
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A hexokinase with broad sugar specificity from a thermophilic bacterium.一种来自嗜热细菌的具有广泛糖特异性的己糖激酶。
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Crystal structures of Escherichia coli ATP-dependent glucokinase and its complex with glucose.大肠杆菌ATP依赖性葡萄糖激酶及其与葡萄糖复合物的晶体结构。
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Bacillus subtilis GlcK activity requires cysteines within a motif that discriminates microbial glucokinases into two lineages.枯草芽孢杆菌葡萄糖激酶（GlcK）的活性需要一个基序内的半胱氨酸，该基序可将微生物葡萄糖激酶分为两个谱系。
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Unique sugar metabolism and novel enzymes of hyperthermophilic archaea.嗜热古菌独特的糖代谢与新型酶类
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7
ATP-dependent glucokinase from the hyperthermophilic bacterium Thermotoga maritima represents an extremely thermophilic ROK glucokinase with high substrate specificity.来自嗜热栖热菌的ATP依赖性葡萄糖激酶是一种具有高底物特异性的极端嗜热ROK葡萄糖激酶。
FEMS Microbiol Lett. 2003 Sep 26;226(2):405-11. doi: 10.1016/S0378-1097(03)00642-6.
8
Crystal structure of an ADP-dependent glucokinase from Pyrococcus furiosus: implications for a sugar-induced conformational change in ADP-dependent kinase.嗜热栖热菌中一种依赖ADP的葡萄糖激酶的晶体结构：对糖诱导的依赖ADP激酶构象变化的启示
J Mol Biol. 2003 Aug 22;331(4):871-83. doi: 10.1016/s0022-2836(03)00792-7.
9
The hexokinase of the hyperthermophile Thermoproteus tenax. ATP-dependent hexokinases and ADP-dependent glucokinases, teo alternatives for glucose phosphorylation in Archaea.嗜热栖热菌的己糖激酶。ATP 依赖性己糖激酶和 ADP 依赖性葡萄糖激酶，古菌中葡萄糖磷酸化的两种替代方式。
J Biol Chem. 2003 May 23;278(21):18744-53. doi: 10.1074/jbc.M301914200. Epub 2003 Mar 7.
10
The first archaeal ATP-dependent glucokinase, from the hyperthermophilic crenarchaeon Aeropyrum pernix, represents a monomeric, extremely thermophilic ROK glucokinase with broad hexose specificity.首个来自嗜热泉古菌栖热袍菌的古菌ATP依赖性葡萄糖激酶，是一种具有广泛己糖特异性的单体、极端嗜热的ROK葡萄糖激酶。
J Bacteriol. 2002 Nov;184(21):5955-65. doi: 10.1128/JB.184.21.5955-5965.2002.

嗜热栖热菌一种假定的葡萄糖激酶/己糖激酶的结晶及初步晶体学分析

Crystallization and preliminary crystallographic analysis of a putative glucokinase/hexokinase from Thermus thermophilus.

作者信息

Nakamura Tsutomu, Kashima Yasuhiro, Mine Shouhei, Oku Takashi, Uegaki Koichi

机构信息

National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1559-62. doi: 10.1107/S1744309111041145. Epub 2011 Nov 25.

DOI:10.1107/S1744309111041145
PMID:22139166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3232139/
Abstract

Glucokinase/hexokinase catalyzes the phosphorylation of glucose to glucose 6-phosphate, which is the first step of glycolysis. The open reading frame TTHA0299 of the extreme thermophile Thermus thermophilus encodes a putative glucokinase/hexokinase which contains the consensus sequence for proteins from the repressors, open reading frames and sugar kinases family. In this study, the glucokinase/hexokinase from T. thermophilus was purified and crystallized using polyethylene glycol 8000 as a precipitant. Diffraction data were collected and processed to 2.02 Å resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 70.93, b = 138.14, c = 75.16 Å, β = 95.41°.

摘要

葡萄糖激酶/己糖激酶催化葡萄糖磷酸化生成6-磷酸葡萄糖，这是糖酵解的第一步。嗜热栖热菌（Thermus thermophilus）的开放阅读框TTHA0299编码一种假定的葡萄糖激酶/己糖激酶，它包含来自阻遏物、开放阅读框和糖激酶家族蛋白质的共有序列。在本研究中，使用聚乙二醇8000作为沉淀剂对嗜热栖热菌的葡萄糖激酶/己糖激酶进行了纯化和结晶。收集了衍射数据并处理至2.02 Å分辨率。晶体属于空间群P2(1)，晶胞参数为a = 70.93，b = 138.14，c = 75.16 Å，β = 95.41°。