灵芝漆酶的纯化、结晶及初步X射线结构分析
Purification, crystallization and preliminary X-ray structure analysis of the laccase from Ganoderma lucidum.
作者信息
Lyashenko Andrey V, Belova Oksana, Gabdulkhakov Azat G, Lashkov Alexander A, Lisov Alexandr V, Leontievsky Alexey A, Mikhailov Al'bert M
机构信息
A V Shubnikov Institute of Crystallography, Russian Academy of Sciences, Moscow, Russia.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):926-9. doi: 10.1107/S1744309111021129. Epub 2011 Jul 20.
Abstract
The ligninolytic enzymes of the basidiomycetes play a key role in the global carbon cycle. A characteristic property of these enzymes is their broad substrate specificity, which has led to their use in various biotechnologies, thus stimulating research into the three-dimensional structures of ligninolytic enzymes. This paper presents the purification, crystallization and preliminary X-ray analysis of the laccase from the ligninolytic basidiomycete Ganoderma lucidum.
摘要
担子菌的木质素分解酶在全球碳循环中起着关键作用。这些酶的一个显著特性是其广泛的底物特异性，这使得它们在各种生物技术中得到应用，从而推动了对木质素分解酶三维结构的研究。本文介绍了木质素分解担子菌灵芝漆酶的纯化、结晶及初步X射线分析。
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