Steven F S, Maier H, Arndt J, Born I A
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U,K.
Cancer Lett. 1990 Apr 30;50(3):191-6. doi: 10.1016/0304-3835(90)90264-x.
We have studied the enzymic status of a tumour associated protease on human colonic tumour cells cultured in vitro and grown in vivo in nude mice. The cultured tumour cells lack this protease. The cells of the tumour colonies in the mice possess active enzyme although the tissue contains an inhibitor capable of inactivating this tumour cell protease. The evidence indicates that the induction of this protease on the tumour cells is a consequence of implantation of the cultured cells into the nude mouse.
我们研究了一种肿瘤相关蛋白酶在体外培养并在裸鼠体内生长的人结肠肿瘤细胞上的酶学状态。培养的肿瘤细胞缺乏这种蛋白酶。小鼠体内肿瘤集落的细胞具有活性酶，尽管该组织含有一种能够使这种肿瘤细胞蛋白酶失活的抑制剂。证据表明，这种蛋白酶在肿瘤细胞上的诱导是将培养细胞植入裸鼠的结果。
