Steven F S, Maier H, Arndt J
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
J Enzyme Inhib. 1989;3(2):145-57. doi: 10.3109/14756368909030373.
The regulation of activity of a protease on the surface of human colonic tumour cells implanted in nude mice has been studied. A synthetic fluorescent probe was used to locate cells possessing active guanidinobenzoatase in frozen sections of tumour bearing tissues. These studies demonstrated the presence of intracellular protein inhibitors of guanidinobenzoatase which could be extracted in isotonic saline and transferred to the outer surface of the tumour cells. This study showed that the inhibition of guanidinobenzoatase was pH dependent and that the tumour cell may regulate the activity of its surface guanidinobenzoatase by exporting lactic acid.
对裸鼠体内植入的人结肠肿瘤细胞表面蛋白酶活性的调节进行了研究。使用一种合成荧光探针在荷瘤组织的冰冻切片中定位具有活性胍基苯甲酸酶的细胞。这些研究表明存在胍基苯甲酸酶的细胞内蛋白抑制剂，其可在等渗盐水中提取并转移至肿瘤细胞的外表面。该研究表明胍基苯甲酸酶的抑制作用是pH依赖性的，并且肿瘤细胞可能通过输出乳酸来调节其表面胍基苯甲酸酶的活性。
