Yu Yuan, Xiao Chaowen, Chen Kun, Zheng Jianwei, Zhang Jun, Zhao Xinyang, Xue Xinbo
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):515. doi: 10.1007/s11596-011-0482-4. Epub 2011 Aug 7.
There has been an ongoing search for clinically acceptable methods for the accurate, efficient and simple diagnosis and prognosis of hepatocellular carcinoma (HCC). Optical spectroscopy is a technique with potential clinical applications to diagnose cancer diseases. The purpose of this study was to obtain the optical properties of HCC tissues and non-tumorous hepatic tissues and identify the difference between them. A total of 55 tissue samples (HCC tissue, n=38; non-tumorous hepatic tissue, n=17) were surgically resected from patients with HCC. The optical parameters were measured in 10-nm steps using single-integrating-sphere system in the wavelength range of 400 to 1800 nm. It was found that the optical properties and their differences varied with the wavelength for the HCC tissue and the non-tumorous hepatic tissue in the entire wavelength range of research. The absorption coefficient of the HCC tissue (1.48±0.99, 1.46±0.88, 0.86±0.61, 2.15±0.53, 0.54±0.10, 0.79±0.15 mm(-1)) was significantly lower than that of the non-tumorous hepatic tissue (2.79±1.73, 3.13±1.47, 3.06±2.79, 2.57±0.55, 0.62±0.10, 0.93±0.16 mm(-1)) at wavelengths of 400, 410, 450, 1450, 1660 and 1800 nm, respectively (P<0.05). The reduced scattering coefficient of HCC tissue (5.28±1.70, 4.91±1.54, 1.26±0.35 mm(-1)) and non-tumorous hepatic tissue (8.14±3.70, 9.27±3.08, 2.55±0.57 mm(-1)) was significantly different at 460, 500 and 1800 nm respectively (P<0.05). These results show different pathologic liver tissues have different optical properties. It provides a better understanding of the relationship between optical parameters and physiological characteristics in human liver tissues. And it would be very useful for developing a non-invasive, real-time, simple and efficient way for medical management of HCC in the future.
一直在寻找临床上可接受的用于准确、高效且简单地诊断和预测肝细胞癌（HCC）的方法。光谱学是一种具有潜在临床应用价值以诊断癌症疾病的技术。本研究的目的是获取HCC组织和非肿瘤性肝组织的光学特性，并确定它们之间的差异。总共从HCC患者身上手术切除了55个组织样本（HCC组织，n = 38；非肿瘤性肝组织，n = 17）。使用单积分球系统在400至1800 nm波长范围内以10 nm步长测量光学参数。发现在整个研究波长范围内，HCC组织和非肿瘤性肝组织的光学特性及其差异随波长而变化。在400、410、450、1450、1660和1800 nm波长处，HCC组织的吸收系数（1.48±0.99、1.46±0.88、0.86±0.61、2.15±0.53、0.54±0.10、0.79±0.15 mm⁻¹）分别显著低于非肿瘤性肝组织（2.79±1.73、3.13±1.47、3.06±2.79、2.57±0.55、0.62±0.10、0.93±0.16 mm⁻¹）（P<0.05）。HCC组织（5.28±1.70、4.91±1.54、1.26±0.35 mm⁻¹）和非肿瘤性肝组织（8.14±3.70、9.27±3.08、2.55±0.57 mm⁻¹）的约化散射系数在460、500和1800 nm处分别有显著差异（P<0.05）。这些结果表明不同病理状态的肝组织具有不同的光学特性。这有助于更好地理解人体肝组织中光学参数与生理特征之间的关系。并且这对于未来开发一种用于HCC医疗管理的非侵入性、实时、简单且高效的方法将非常有用。
