[Effect of separation and purification processes on extraction ratio of polysaccharides in Rabdosia rubescens and immunocompetence].

OBJECTIVE

To select a good method for purifying polysaccharides from Rabdosia rubescens, and study their immunological activity.

METHODS

DEAE-Sepharose Fast Flow (Cl-) weak polar anion-exchange chromatography and stepwise ethanol precipitation were used to isolate and purify the polysaccharides from Rabdosia rubescens, respectively. MTT method was used to evaluate the immune activity.

RESULTS

Five components were obtained from DEAE-Sepharose Fast Flow (Cl-) weak polar anion-exchange chromatography and four polysaccharides were obtained through alcohol precipitation.

CONCLUSION

Polysaccharides from Rabdosia rubescens by stepwise ethanol precipitation show stronger immunological activity. Their immunomodulatory effect and related mechanism will be researched further.