Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis, IN 46202-4887, USA.
Pharmacol Biochem Behav. 2011 Nov;100(1):90-7. doi: 10.1016/j.pbb.2011.07.017. Epub 2011 Jul 29.
Alcohol binge-drinking, especially among adolescents and young adults, is a serious public health concern. The present study examined ethanol binge-like drinking by peri-adolescent [postnatal days (PNDs 30-72)] and adult (PNDs 90-132) alcohol-preferring (P) rats with a drinking-in-the-dark-multiple-scheduled-access (DID-MSA) procedure used by our laboratory. Male and female P rats were provided concurrent access to 15% and 30% ethanol for three 1-h sessions across the dark cycle 5 days/week. For the 1st week, adolescent and adult female P rats consumed 3.4 and 1.6g/kg of ethanol, respectively, during the 1st hour of access, whereas for male rats the values were 3.5 and 1.1g/kg of ethanol, respectively. Adult intakes increased to ~2.0 g/kg/h and adolescent intakes decreased to ~2.5 g/kg/h across the 6 weeks of ethanol access. The daily ethanol intake of adult DID-MSA rats approximated or modestly exceeded that seen in continuous access (CA) rats or the selection criterion for P rats (≥5 g/kg/day). However, in general, the daily ethanol intake of DID-MSA peri-adolescent rats significantly exceeded that of their CA counterparts. BELs were assessed at 15-min intervals across the 3rd hour of access during the 4th week. Ethanol intake was 1.7 g/kg vs. 2.7 g/kg and BELs were 57 mg% vs. 100mg% at 15- and 60-min, respectively. Intoxication induced by DID-MSA in female P rats was assessed during the 1st vs. 4th week of ethanol access. Level of impairment did not differ between the 2 weeks (106 vs. 97 s latency to fall, 120 s criterion) and was significant (vs. naïve controls) only during the 4th week. Overall, these findings support the use of the DID-MSA procedure in rats, and underscore the presence of age- and sex-dependent effects mediating ethanol binge-like drinking in P rats.
酒精 binge 饮酒，尤其是在青少年和年轻人中，是一个严重的公共卫生问题。本研究通过我们实验室使用的暗时多相序进（DID-MSA）程序，检测了围青春期（出生后第 30-72 天）和成年（第 90-132 天）酒精偏好（P）大鼠的乙醇 binge 样饮酒。雄性和雌性 P 大鼠在暗周期内的 5 天/周内，同时提供 15%和 30%乙醇，进行 3 次 1 小时的访问。在第 1 周，青春期和成年雌性 P 大鼠在第 1 小时内分别摄入 3.4 和 1.6g/kg 的乙醇，而雄性大鼠的摄入量分别为 3.5 和 1.1g/kg 的乙醇。在 6 周的乙醇摄入期间，成年大鼠的摄入量增加到约 2.0g/kg/h，而青春期大鼠的摄入量减少到约 2.5g/kg/h。成年 DID-MSA 大鼠的每日乙醇摄入量接近或略高于连续摄入（CA）大鼠或 P 大鼠的选择标准（≥5g/kg/天）。然而，一般来说，DID-MSA 围青春期大鼠的每日乙醇摄入量明显高于 CA 对照大鼠。在第 4 周的第 3 小时访问期间，每隔 15 分钟评估 BEL。在 15 分钟和 60 分钟时，乙醇摄入量分别为 1.7g/kg 和 2.7g/kg，BEL 分别为 57mg%和 100mg%。在第 1 周和第 4 周的乙醇摄入期间评估 DID-MSA 在雌性 P 大鼠中引起的中毒。在这 2 周内，损伤程度没有差异（106 与 97 秒跌倒潜伏期，120 秒标准），仅在第 4 周时与未处理的对照相比具有显著性（106 与 97 秒跌倒潜伏期，120 秒标准）。总体而言，这些发现支持在大鼠中使用 DID-MSA 程序，并强调了年龄和性别依赖性效应在 P 大鼠乙醇 binge 样饮酒中的存在。
