Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Biotechnol Lett. 2011 Dec;33(12):2457-61. doi: 10.1007/s10529-011-0719-0. Epub 2011 Aug 9.
Fusarium moniliforme var. subglutinans was selected from among 100 strains of fungi for producing ginsenoside F(1) from ginsenoside Rg(1). The enzyme responsible was purified as a single 85 kDa band with a specific activity of 136 U mg(-1). It hydrolysed glucose-linked ginsenosides Rb(1), Rd and Rg(1) but not for other monosaccharide-linked ginsenosides, Rb(2), Rc, R(1), and Re. Under the optimum conditions of pH 6.0, 50 °C, 30 U l(-1) of enzyme, and 5 mg Rg(1) ml(-1), 4 mg F(1) ml(-1) was produced after 4 h, with a molar yield of 100% and a productivity of 1 g l(-1) h(-1). This represents the highest productivity and conversion yield of F(1) yet reported.
从 100 株真菌中筛选出串珠镰刀菌（Fusarium moniliforme var. subglutinans），用于从人参皂苷 Rg(1)生产人参皂苷 F(1)。负责该酶的酶被纯化为具有 136 U mg(-1)比活性的单一 85 kDa 带。它水解葡萄糖连接的人参皂苷 Rb(1)、Rd 和 Rg(1)，但不水解其他单糖连接的人参皂苷 Rb(2)、Rc、R(1)和 Re。在 pH 6.0、50°C、30 U l(-1)的酶和 5 mg Rg(1) ml(-1)的最佳条件下，4 h 后可产生 4 mg F(1) ml(-1)，摩尔产率为 100%，产率为 1 g l(-1) h(-1)。这代表了迄今为止报道的最高的 F(1)生产力和转化率。
