Wang Yu, Choi Kang-Duk, Yu Hongshan, Jin Fengxie, Im Wan-Taek
College of Biotechnology, Dalian Polytechnic University, Ganjingzi-qu, Dalian, PR China; Department of Biotechnology, Hankyong National University, Anseong, Korea.
Genomic Informatics Center, Graduate School of Future Convergence Technology, Hankyong National University, Anseong, Korea.
J Ginseng Res. 2016 Apr;40(2):121-6. doi: 10.1016/j.jgr.2015.06.003. Epub 2015 Jun 19.
Ginsenoside F1, a pharmaceutical component of ginseng, is known to have antiaging, antioxidant, anticancer, and keratinocyte protective effects. However, the usage of ginsenoside F1 is restricted owing to the small amount found in Korean ginseng.
To enhance the production of ginsenoside F1 as a 10 g unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the commercial enzyme Cellulase KN from Aspergillus niger with food grade, which has ginsenoside-transforming ability. The proposed optimum reaction conditions of Cellulase KN were pH 5.0 and 50°C.
Cellulase KN could effectively transform the ginsenosides Re and Rg1 into F1. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 5.0 and 50°C for 48 h with protopanaxatriol-type ginsenoside mixture (at a concentration of 10 mg/mL) from ginseng roots. Finally, 13.0 g of F1 was produced from 50 g of protopanaxatriol-type ginsenoside mixture with 91.5 ± 1.1% chromatographic purity.
The results suggest that this enzymatic method could be exploited usefully for the preparation of ginsenoside F1 to be used in cosmetic, functional food, and pharmaceutical industries.
人参皂苷F1是人参的一种药用成分，已知具有抗衰老、抗氧化、抗癌和保护角质形成细胞的作用。然而，由于在高丽参中含量较少，人参皂苷F1的应用受到限制。
为了以高特异性、产率和纯度大量生产10克单位的人参皂苷F1，开发了一种酶促生物转化方法，采用食品级的黑曲霉商业酶纤维素酶KN，其具有人参皂苷转化能力。所提出的纤维素酶KN的最佳反应条件为pH 5.0和50°C。
纤维素酶KN可有效地将人参皂苷Re和Rg1转化为人参皂苷F1。在10 L罐式发酵罐中，于pH 5.0和50°C下，用人参根中的原人参三醇型人参皂苷混合物（浓度为10 mg/mL）进行放大生物转化反应48小时。最后，从50克原人参三醇型人参皂苷混合物中生产出13.0克人参皂苷F1，色谱纯度为91.5±1.1%。
结果表明，这种酶促方法可有效地用于制备人参皂苷F1，用于化妆品、功能性食品和制药行业。
