Braun R, Doerr H W, Geisen H P, Hornig C, Huschka U, Munk K
J Med Virol. 1981;8(3):207-14. doi: 10.1002/jmv.1890080308.
The rubella specific IgM titer in the serum specimens originating from healthy persons and from patients with clinical signs of rubella infection was determined by hemagglutination inhibition or hemagglutination reduction after IgM separation with the following methods: (a) density gradient centrifugation; (b) polyacrylamide agarose gel chromatography; (c) ion exchange chromatography with diethylaminoethyl cellulose columns; (d) solid-phase immunosorbent technique using microplates; (e) solid-phase immunosorbent technique using polyacrylamide microimmunobeads. Alternatively, we removed IgG and IgA by the use of protein A, anti-IgG, and anti-IgA, covalently coupled to controlled-pore glass (f). The titers obtained by the different methods showed qualitatively good correlations when combined with mercaptoethanol reduction. The quantitative measurement of specific IgM titers, however, revealed a lower sensitivity of column chromatography and methods of removal of IgG/IgA.
采用以下方法分离IgM后，通过血凝抑制或血凝减少法测定来自健康人和有风疹感染临床症状患者血清标本中的风疹特异性IgM滴度：(a)密度梯度离心法；(b)聚丙烯酰胺琼脂糖凝胶色谱法；(c)用二乙氨基乙基纤维素柱进行离子交换色谱法；(d)使用微孔板的固相免疫吸附技术；(e)使用聚丙烯酰胺微免疫珠的固相免疫吸附技术。另外，我们通过使用与可控孔径玻璃共价偶联的蛋白A、抗IgG和抗IgA去除IgG和IgA(f)。当与巯基乙醇还原法结合使用时，不同方法获得的滴度在质量上显示出良好的相关性。然而，特异性IgM滴度的定量测量显示柱色谱法以及去除IgG/IgA的方法灵敏度较低。
