Kalimo K O, Meurman O H, Halonen P E, Ziola B R, Viljanen M K, Granfors K, Toivanen P
J Clin Microbiol. 1976 Aug;4(2):117-23. doi: 10.1128/jcm.4.2.117-123.1976.
A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.
已开发出一种固相放射免疫测定方法，用于检测人血清标本中的风疹病毒特异性免疫球蛋白G（IgG）和IgM抗体。将纯化的风疹病毒吸附到聚苯乙烯球上，通过随后结合125I标记的抗人γ或抗人μ免疫球蛋白来检测附着在病毒处理过的球上的抗体。共检测了77份血清标本。在IgG测定中，阳性和阴性血清之间的结合率高达22，但在IgM测定中很少超过3。发现IgG测定的灵敏度比风疹病毒血凝抑制试验高16至256倍。IgG放射免疫测定可很容易地用于常规诊断。然而，IgM放射免疫测定由于其较低的灵敏度，在常规应用之前必须进行改进。
