风疹病毒免疫球蛋白G和免疫球蛋白M抗体的固相放射免疫测定
Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.
作者信息
Kalimo K O, Meurman O H, Halonen P E, Ziola B R, Viljanen M K, Granfors K, Toivanen P
出版信息
J Clin Microbiol. 1976 Aug;4(2):117-23. doi: 10.1128/jcm.4.2.117-123.1976.
Abstract
A solid-phase radioimmunoassay method has been developed for the detection of rubella virus-specific immunoglobulin G (IgG) and IgM antibodies in human serum specimens. Purified rubella virus was adsorbed onto polystyrene balls, and antibodies that attached to the virus-treated balls were detected by subsequent binding of 125I-labeled anti-human gamma or anti-human mu immunoglobulins. A total of 77 serum specimens were tested. Binding ratios between positive and negative sera were as high as 22 in the IgG assay but rarely exceeded 3 in the IgM assay. The sensitivity of the IgG assay was found to be 16 to 256 times higher than that of the rubella virus hemagglutination inhibition test. The IgG radioimmunoassay can be readily adopted for routine diagnostic use. The IgM radioimmunoassay, however, due to its lower sensitivity, must be modified before being routinely applied.
摘要
已开发出一种固相放射免疫测定方法，用于检测人血清标本中的风疹病毒特异性免疫球蛋白G（IgG）和IgM抗体。将纯化的风疹病毒吸附到聚苯乙烯球上，通过随后结合125I标记的抗人γ或抗人μ免疫球蛋白来检测附着在病毒处理过的球上的抗体。共检测了77份血清标本。在IgG测定中，阳性和阴性血清之间的结合率高达22，但在IgM测定中很少超过3。发现IgG测定的灵敏度比风疹病毒血凝抑制试验高16至256倍。IgG放射免疫测定可很容易地用于常规诊断。然而，IgM放射免疫测定由于其较低的灵敏度，在常规应用之前必须进行改进。
相似文献
1
Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.风疹病毒免疫球蛋白G和免疫球蛋白M抗体的固相放射免疫测定
J Clin Microbiol. 1976 Aug;4(2):117-23. doi: 10.1128/jcm.4.2.117-123.1976.
2
Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test.风疹病毒免疫球蛋白M抗体的固相放射免疫测定：与蔗糖密度梯度离心试验的比较
J Clin Microbiol. 1977 Mar;5(3):257-62. doi: 10.1128/jcm.5.3.257-262.1977.
3
Solid-phase radioimmunoassay of IgA, IgG, and IgM antibodies to human rotavirus.人轮状病毒IgA、IgG和IgM抗体的固相放射免疫测定
J Med Virol. 1979;3(4):281-9. doi: 10.1002/jmv.1890030406.
4
Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies.检测风疹免疫球蛋白M和免疫球蛋白A抗体的快速方法。
J Clin Microbiol. 1975 Feb;1(2):132-5. doi: 10.1128/jcm.1.2.132-135.1975.
5
[Use of a rapid method for determining primary infection by rubella virus].[一种用于确定风疹病毒原发性感染的快速方法的应用]
Rev Argent Microbiol. 1987 Oct-Dec;19(4):153-9.
6
[Solid-phase enzyme immunoassay for specific immunoglobulin G and immunoglobulin M antibodies of rubella virus (author's transl)].
Ann Microbiol (Paris). 1977 Nov-Dec;128B(4):531-40.
7
A rountine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus.风疹病毒IgA和IgM抗体的常规诊断试验：用金黄色葡萄球菌吸收IgG
J Infect Dis. 1974 Sep;130(3):268-73. doi: 10.1093/infdis/130.3.268.
8
Detection of measles, mumps, and rubella antibodies in saliva using antibody capture radioimmunoassay.采用抗体捕获放射免疫分析法检测唾液中的麻疹、腮腺炎和风疹抗体。
J Med Virol. 1993 Jul;40(3):235-40. doi: 10.1002/jmv.1890400312.
9
Solid-phase radioimmunoassay of herpes simplex virus IgG and IgM antibodies.单纯疱疹病毒IgG和IgM抗体的固相放射免疫测定
J Immunol Methods. 1977;14(2):183-95. doi: 10.1016/0022-1759(77)90008-4.
10
Hemadsorption immunosorbent technique for determination of rubella immunoglobulin M antibody.用于测定风疹免疫球蛋白M抗体的血细胞吸附免疫吸附技术
J Clin Microbiol. 1981 Mar;13(3):410-5. doi: 10.1128/jcm.13.3.410-415.1981.
引用本文的文献
1
Comparison of hemagglutination inhibition test and enzyme-linked immunosorbent assay for determining antibody to rubella virus.血凝抑制试验与酶联免疫吸附测定法在风疹病毒抗体检测中的比较
J Clin Microbiol. 1981 May;13(5):850-4. doi: 10.1128/jcm.13.5.850-854.1981.
2
Rubella antibody determination from heparinised finger-tip blood by single radial haemolysis and enzyme immunoassay.通过单向辐射溶血法和酶免疫测定法从肝素化指尖血中测定风疹抗体。
J Clin Pathol. 1980 Sep;33(9):845-7. doi: 10.1136/jcp.33.9.845.
3
Evaluation of a latex agglutination test for detection of antibodies to rubella virus in selected sera.对用于检测特定血清中风疹病毒抗体的乳胶凝集试验的评估。
J Clin Microbiol. 1983 Jul;18(1):197-8. doi: 10.1128/jcm.18.1.197-198.1983.
4
Enzyme-linked immunosorbent assay for immunoglobulin G and M antibodies to Chlamydia trachomatis in human sera.人血清中沙眼衣原体免疫球蛋白G和M抗体的酶联免疫吸附测定
J Clin Microbiol. 1983 May;17(5):848-52. doi: 10.1128/jcm.17.5.848-852.1983.
5
Subcellular fractions in rubella immunoassays.风疹免疫测定中的亚细胞组分。
Eur J Clin Microbiol. 1984 Feb;3(1):19-24. doi: 10.1007/BF02032809.
6
Rubella serology: a comparison of four methods for exclusion of non-specific serum inhibitors.风疹血清学：四种排除非特异性血清抑制剂方法的比较
J Hyg (Lond). 1981 Jun;86(3):315-27. doi: 10.1017/s0022172400069060.
7
Immunoglobulins M and G to varicella-zoster virus measured by solid-phase radioimmunoassay: antibody responses to varicella and herpes zoster infections.通过固相放射免疫测定法检测的针对水痘-带状疱疹病毒的免疫球蛋白M和G：对水痘和带状疱疹感染的抗体反应
J Clin Microbiol. 1980 Sep;12(3):367-74. doi: 10.1128/jcm.12.3.367-374.1980.
8
Detection of enterovirus specific IgG and IgM antibodies in humans by an indirect solid phase radioimmunoassay.用间接固相放射免疫测定法检测人体中的肠道病毒特异性IgG和IgM抗体。
Med Microbiol Immunol. 1980;168(3):159-71. doi: 10.1007/BF02122850.
9
Enzyme-linked immunosorbent assay for mumps and parainfluenza type 1 immunoglobulin G and immunoglobulin M antibodies.用于检测腮腺炎及1型副流感病毒免疫球蛋白G和免疫球蛋白M抗体的酶联免疫吸附测定
J Clin Microbiol. 1980 Apr;11(4):319-23. doi: 10.1128/jcm.11.4.319-323.1980.
10
Recent rubella virus infection indicated by a low avidity of specific IgG.特异性IgG亲和力低提示近期感染风疹病毒。
J Clin Immunol. 1988 May;8(3):214-21. doi: 10.1007/BF00917569.
本文引用的文献
1
Preparation of iodine-131 labelled human growth hormone of high specific activity.高比活度碘-131标记人生长激素的制备
Nature. 1962 May 5;194:495-6. doi: 10.1038/194495a0.
2
Application of a microtechnique to viral serological investigations.一种微技术在病毒血清学研究中的应用。
J Immunol. 1962 Mar;88:320-9.
3
A serological method for demonstrating recent infection by rubella virus.一种用于证明风疹病毒近期感染的血清学方法。
Br Med J. 1967 Jul 29;3(5560):285-6. doi: 10.1136/bmj.3.5560.285.
4
Rubella hemagglutinin prepared in serum free suspension culture of BHK-21 cells.在BHK - 21细胞无血清悬浮培养中制备的风疹血凝素。
Ann Med Exp Biol Fenn. 1967;45(2):182-5.
5
Rubella complement fixing antigen prepared by alkaline extraction of virus grown in suspension culture of BHK-211 cells.风疹补体结合抗原，通过对在BHK - 211细胞悬浮培养物中生长的病毒进行碱性提取制备而成。
Proc Soc Exp Biol Med. 1967 May;125(1):167-72. doi: 10.3181/00379727-125-32039.
6
Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column.通过在葡聚糖凝胶G - 200柱上对抗体进行分级分离对近期风疹进行单样本诊断。
J Clin Pathol. 1971 Sep;24(6):547-50. doi: 10.1136/jcp.24.6.547.
7
8
Immunoglobulin class of influenza antibodies investigated by radioimmunoassay (RIA).通过放射免疫测定法（RIA）研究的流感抗体的免疫球蛋白类别。
J Immunol. 1972 Oct;109(4):849-56.
9
Mumps class-specific immunoglobulins in radioimmunoassay and conventional serology.放射免疫分析和传统血清学中的腮腺炎类特异性免疫球蛋白
Infect Immun. 1973 Mar;7(3):380-5. doi: 10.1128/iai.7.3.380-385.1973.
10
IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique.用免疫荧光技术测定急性风疹中的IgG、IgA和IgM反应。
J Hyg (Lond). 1972 Sep;70(3):473-85. doi: 10.1017/s0022172400063063.
Zotero 插件