Danielsson B, Ekman R, Johansson B G, Nilsson-Ehle P, Petersson B G
Scand J Clin Lab Invest Suppl. 1978;150:214-7.
A high density lipoprotein (HDL) fraction, designated cholestatic HDL1, has been isolated by zonal ultracentrifugation from patients with long-standing biliary obstruction. This lipoprotein fraction consists exclusively of disc-shaped particles rich in phospholipid and unesterified cholesterol. The fatty acid composition of the minor cholesteryl ester fraction was characterized by a low content of linoleic acid. Apoprotein E was the dominating protein' component, while only small amounts of apolipoprotein A-I were found. Immunochemical studies of cholestatic HDL1 indicate that a considerable proportion of the particles contained apo E as its only protein constituent. All patients had a markedly reduced endogenous cholesterol-esterifying ability. The finding of apparently primary LpE particles in biliary obstruction may be related to this secondary LCAT deficiency.
已通过区带超速离心法从长期胆汁淤积性梗阻患者中分离出一种高密度脂蛋白（HDL）组分，命名为胆汁淤积性HDL1。该脂蛋白组分仅由富含磷脂和未酯化胆固醇的盘状颗粒组成。少量胆固醇酯组分的脂肪酸组成特点是亚油酸含量低。载脂蛋白E是主要的蛋白质成分，而仅发现少量载脂蛋白A-I。对胆汁淤积性HDL1的免疫化学研究表明，相当一部分颗粒仅含有载脂蛋白E作为其蛋白质成分。所有患者的内源性胆固醇酯化能力均明显降低。在胆汁淤积中发现明显的原发性LpE颗粒可能与这种继发性卵磷脂胆固醇酰基转移酶（LCAT）缺乏有关。
