Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie, Hans-Knöll-Institut, Jena, Germany.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1402-1408. doi: 10.1099/ijs.0.033431-0. Epub 2011 Aug 5.
Three Gram-staining-negative non-endospore-forming strains were isolated from farmed fish in Chile: one (LM-09-Fp(T)) from a rainbow trout (Oncorhynchus mykiss) and the others (LM-19-Fp(T) and LM-20-Fp) from two Atlantic salmon (Salmo salar). Phylogenetic analyses based on 16S rRNA gene sequences indicated that all three isolates belonged to the genus Flavobacterium. In these analyses, strain LM-09-Fp(T) appeared most closely related to the type strains of Flavobacterium chungangense (98.5 % sequence similarity), Flavobacterium glaciei (98.2 %), Flavobacterium aquidurense (97.6 %), Flavobacterium saccharophilum (97.6 %) and Flavobacterium hercynium (97.6 %). The 16S rRNA gene sequences of strains LM-19-Fp(T) and LM-20-Fp were found to be identical and most similar to the corresponding sequences of the type strains of Flavobacterium aquidurense (98.6 %), Flavobacterium frigidimaris (98.5 %), Flavobacterium hercynium (97.9 %), Flavobacterium saccharophilum (97.7 %) and Flavobacterium pectinovorum (97.7 %). For each of the three novel strains, menaquinone (MK-6) was the predominant respiratory quinone and the major compounds in the polar lipid profile were phosphatidylethanolamine, an unidentified aminolipid, phosphatidylserine and two or three unknown lipids. The fatty acid profile of each strain, which comprised major amounts of iso-C(15:0), C(15:0) and summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) as well as smaller amounts of various hydroxylated fatty acids (e.g. iso-C(16:0) 3-OH, iso-C(17:0) 3-OH, C(16:0) 3-OH and C(15:0) 3-OH), indicated that each belonged to the genus Flavobacterium. Based on their physiological and biochemical characteristics and the results of DNA-DNA hybridizations, which showed relatively low levels of relatedness between the novel strains and the most closely related Flavobacterium species, strain LM-09-Fp(T) ( = LMG 26360(T) = CCM 7940(T)) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium chilense sp. nov. is proposed, and strains LM-19-Fp(T) ( = LMG 26359(T) = CCM 7939(T)) and LM-20-Fp ( = LMG 26331) represent a second novel species within the same genus, for which the name Flavobacterium araucananum sp. nov. is proposed.
一株（LM-09-Fp(T)）来自虹鳟（Oncorhynchus mykiss），另两株（LM-19-Fp(T)和 LM-20-Fp）来自大西洋鲑（Salmo salar）。基于 16S rRNA 基因序列的系统发育分析表明，这三株菌均属于黄杆菌属。在这些分析中，菌株 LM-09-Fp(T)与 Flavobacterium chungangense（98.5%序列相似性）、Flavobacterium glaciei（98.2%）、Flavobacterium aquidurense（97.6%）、Flavobacterium saccharophilum（97.6%）和 Flavobacterium hercynium（97.6%）的模式菌株最为密切相关。菌株 LM-19-Fp(T)和 LM-20-Fp 的 16S rRNA 基因序列完全相同，与 Flavobacterium aquidurense（98.6%）、Flavobacterium frigidimaris（98.5%）、Flavobacterium hercynium（97.9%）、Flavobacterium saccharophilum（97.7%）和 Flavobacterium pectinovorum（97.7%）的模式菌株最为相似。对于这三个新菌株，menaquinone（MK-6）是主要的呼吸醌，极性脂分析中的主要成分是磷脂酰乙醇胺、一种未鉴定的胺脂、磷脂酰丝氨酸和两种或三种未知脂质。每个菌株的脂肪酸谱都以 iso-C(15:0)、C(15:0) 和总和特征 3（C(16:1)ω7c 和/或 iso-C(15:0) 2-OH）为主，同时还有少量各种羟基脂肪酸（如 iso-C(16:0) 3-OH、iso-C(17:0) 3-OH、C(16:0) 3-OH 和 C(15:0) 3-OH），表明它们都属于黄杆菌属。根据它们的生理生化特性和 DNA-DNA 杂交的结果，这些新菌株与最密切相关的黄杆菌种之间的相关性较低，菌株 LM-09-Fp(T)（=LMG 26360(T)=CCM 7940(T)）代表了黄杆菌属中的一个新种，因此命名为 Flavobacterium chilense sp. nov.，菌株 LM-19-Fp(T)（=LMG 26359(T)=CCM 7939(T)）和 LM-20-Fp（=LMG 26331）代表了该属中的第二个新种，因此命名为 Flavobacterium araucananum sp. nov.。
