Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824, USA.
Int J Syst Evol Microbiol. 2014 Feb;64(Pt 2):406-412. doi: 10.1099/ijs.0.051433-0. Epub 2013 Oct 4.
Two strains (T16(T) and S12) of a Gram-reaction-negative, rod-shaped, yellow-pigmented bacterium were recovered from kidneys of feral spawning adult Chinook salmon (Oncorhynchus tshawytscha) and the gills of captive-reared Chinook salmon fingerlings suffering a mortality episode, respectively. Polyphasic characterization revealed that these strains possessed gliding motility, contained a flexirubin-type pigment, proteolysed multiple substrates (i.e. gelatin, casein and elastin), and had a mean DNA G+C content of 35.6 mol%. Sequencing of the 16S rRNA gene demonstrated that strains T16(T) and S12 were nearly identical to one another (>99% similarity) and were placed within the genus Flavobacterium, with Flavobacterium aquidurense CCUG 59847(T) (98.3%), Flavobaterium araucananum CCUG 61031(T) (98.2%) and Flavobacterium frigidimaris (AB183888, 98.1%) being their closest relatives. Subsequent phylogenetic analyses using neighbour-joining, maximum-parsimony and Bayesian methodologies demonstrated that strains T16(T) and S12 formed a well-supported clade within the genus Flavobacterium that was distinct from other Flavobacterium species. The major fatty acid constituents of strains T16(T) and S12 were iso-C15 : 0, C16 : 1ω6c and/or C16 : 1ω7c, iso-C17 : 0 3-OH, and iso-C15 : 0 3-OH according to fatty acid methyl ester analysis. The mean level of DNA-DNA relatedness between strain T16(T) and F. aquidurense CCUG 59847(T) and F. araucananum CCUG 61031(T) was 23 and 29%, respectively. Thus, the data accumulated in this study support the suggestion that strains T16(T) and S12 represent a novel species of the genus Flavobacterium, for which the name Flavobacterium spartansii sp. nov. is proposed. The type strain is T16(T) ( = LMG 27337(T) = ATCC BAA-2541(T)). Emended descriptions of F. aquidurense and F. araucananum are also proposed.
从野生产卵成年奇努克三文鱼（Oncorhynchus tshawytscha）的肾脏和人工养殖的奇努克三文鱼鱼苗的鳃中分别分离到两种革兰氏反应阴性、杆状、黄色素产生菌的菌株（T16(T)和 S12）。多相特征分析表明，这些菌株具有滑行运动能力，含有一种 flexirubin 型色素，能分解多种底物（即明胶、酪蛋白和弹性蛋白），其 DNA G+C 含量平均值为 35.6 mol%。16S rRNA 基因测序表明，菌株 T16(T)和 S12 彼此非常相似（>99%相似度），并被归入黄杆菌属，与水黄杆菌 CCUG 59847(T)（98.3%）、阿拉斯加黄杆菌 CCUG 61031(T)（98.2%）和嗜冷黄杆菌（AB183888，98.1%）最为接近。随后使用邻接法、最大简约法和贝叶斯法进行的系统发育分析表明，菌株 T16(T)和 S12 在黄杆菌属内形成了一个支持度很高的分支，与其他黄杆菌属物种不同。菌株 T16(T)和 S12 的主要脂肪酸成分根据脂肪酸甲酯分析为 iso-C15:0、C16:1ω6c 和/或 C16:1ω7c、iso-C17:0 3-OH 和 iso-C15:0 3-OH。菌株 T16(T)与水黄杆菌 CCUG 59847(T)和阿拉斯加黄杆菌 CCUG 61031(T)之间的 DNA-DNA 相关水平分别为 23%和 29%。因此，本研究积累的数据支持菌株 T16(T)和 S12 代表黄杆菌属的一个新种的观点，建议将其命名为 Spartansii 属的 Flavobacterium spartansii sp. nov.。模式菌株为 T16(T)（=LMG 27337(T)=ATCC BAA-2541(T)）。还提出了对水黄杆菌和阿拉斯加黄杆菌的修正描述。
