Kämpfer Peter, Irgang Rute, Glaeser Stefanie P, Busse Hans-Jürgen, Criscuolo Alexis, Clermont Dominique, Avendaño-Herrera Ruben
Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
Centro FONDAP, Interdisciplinary Center for Aquaculture Research (INCAR), Viña del Mar, Chile.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6147-6154. doi: 10.1099/ijsem.0.004510.
A Gram-staining-negative non endospore-forming strain, T13(2019) was isolated from water samples from Atlantic salmon () fry culture in Chile and studied in detail for its taxonomic position. The isolate shared highest 16S rRNA gene sequence similarities with the type strains of (98.44 %) followed by and (both 98.22 %). Menaquinone MK-6 was the predominant respiratory quinone in T13(2019). Major polar lipids were phosphatidylethanolamine, an ornithine lipid and the unidentified polar lipids L1, L3 and L4 lacking a functional group. The major polyamine was -homospermidine. The fatty acid profile contained major amounts of iso-C, iso-C 3-OH, iso-C 3-OH, C, summed feature 3 (C c and/or iso-C 2-OH) and various hydroxylated fatty acids in smaller amounts, among them iso-C 3-OH, and C 3-OH, which supported the grouping of the isolate into the genus . Physiological/biochemical characterisation and ANI calculations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation. In addition it became obvious, that the type strains of and showed 100 % 16S rRNA gene sequence similarities and ANI values of 97.21%/ 97.59 % and DDH values of 80.40 % [77.5 and 83%]. These data indicate that and belong to the same species and it is proposed that is a later heterotypic synonym of . For strain T13(2019) (=CIP 111411=LMG 30298=CCM 8798) a new species with the name sp. nov. is proposed.
从智利大西洋鲑鱼苗养殖水样中分离出一株革兰氏染色阴性、不形成芽孢的菌株T13(2019)，并对其分类地位进行了详细研究。该分离株与[具体菌株名称1]的模式菌株16S rRNA基因序列相似度最高(98.44%)，其次是[具体菌株名称2]和具体菌株名称3。甲基萘醌MK-6是T13(2019)中的主要呼吸醌。主要极性脂为磷脂酰乙醇胺、一种鸟氨酸脂以及缺乏官能团的未鉴定极性脂L1、L3和L4。主要多胺为γ-高亚精胺。脂肪酸谱包含大量的异-C15:0、异-C15:0 3-OH、异-C17:0 3-OH、C16:0、总和特征3(C16:1ω7c和/或异-C15:0 2-OH)以及少量各种羟基化脂肪酸，其中异-C15:0 3-OH和C16:0 3-OH支持将该分离株归入[属名]属。通过与最密切相关物种的模式菌株进行生理/生化特征分析和ANI计算，实现了清晰的表型和基因型区分。此外，很明显，[具体菌株名称2]和[具体菌株名称3]的模式菌株16S rRNA基因序列相似度为100%，ANI值分别为97.21%/97.59%，DDH值为80.40%[77.5%和83%]。这些数据表明[具体菌株名称2]和[具体菌株名称3]属于同一物种，因此建议将[具体菌株名称2]作为[具体菌株名称3]的晚出异名。对于菌株T13(2019)(=CIP 111411=LMG 30298=CCM 8798)，提出了一个新物种，命名为[新物种名称] sp. nov.。
