Asharani P V, Lian Wu Yi, Gong Zhiyuan, Valiyaveettil Suresh
Department of Chemistry, Faculty of Science, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Nanotechnology. 2008 Jun 25;19(25):255102. doi: 10.1088/0957-4484/19/25/255102. Epub 2008 May 14.
This study was initiated to enhance our insight on the health and environmental impact of silver nanoparticles (Ag-np). Using starch and bovine serum albumin (BSA) as capping agents, silver nanoparticles were synthesized to study their deleterious effects and distribution pattern in zebrafish embryos (Danio rerio). Toxicological endpoints like mortality, hatching, pericardial edema and heart rate were recorded. A concentration-dependent increase in mortality and hatching delay was observed in Ag-np treated embryos. Additionally, nanoparticle treatments resulted in concentration-dependent toxicity, typified by phenotypes that had abnormal body axes, twisted notochord, slow blood flow, pericardial edema and cardiac arrhythmia. Ag(+) ions and stabilizing agents showed no significant defects in developing embryos. Transmission electron microscopy (TEM) of the embryos demonstrated that nanoparticles were distributed in the brain, heart, yolk and blood of embryos as evident from the electron-dispersive x-ray analysis (EDS). Furthermore, the acridine orange staining showed an increased apoptosis in Ag-np treated embryos. These results suggest that silver nanoparticles induce a dose-dependent toxicity in embryos, which hinders normal development.
开展本研究是为了加深我们对银纳米颗粒（Ag-np）对健康和环境影响的认识。以淀粉和牛血清白蛋白（BSA）作为封端剂，合成银纳米颗粒以研究其在斑马鱼胚胎（Danio rerio）中的有害影响和分布模式。记录了死亡率、孵化率、心包水肿和心率等毒理学终点指标。在经Ag-np处理的胚胎中观察到死亡率呈浓度依赖性增加以及孵化延迟。此外，纳米颗粒处理导致浓度依赖性毒性，表现为身体轴线异常、脊索扭曲、血流缓慢、心包水肿和心律失常等表型。Ag(+)离子和稳定剂在发育中的胚胎中未显示出明显缺陷。胚胎的透射电子显微镜（TEM）检查表明，从电子能谱分析（EDS）可以看出，纳米颗粒分布在胚胎的脑、心脏、卵黄和血液中。此外，吖啶橙染色显示经Ag-np处理的胚胎中凋亡增加。这些结果表明，银纳米颗粒在胚胎中诱导剂量依赖性毒性，阻碍正常发育。
