Bickford Lissett, Sun Jiantang, Fu Kun, Lewinski Nastassja, Nammalvar Vengadesan, Chang Joseph, Drezek Rebekah
Department of Bioengineering, Rice University, Houston, TX 77005, USA.
Nanotechnology. 2008 Aug 6;19(31):315102. doi: 10.1088/0957-4484/19/31/315102. Epub 2008 Jun 24.
We demonstrate the capability of using immunotargeted gold nanoshells as contrast agents for in vitro two-photon microscopy. The two-photon luminescence properties of different-sized gold nanoshells are first validated using near-infrared excitation at 780 nm. The utility of two-photon microscopy as a tool for imaging live HER2-overexpressing breast cancer cells labeled with anti-HER2-conjugated nanoshells is then explored and imaging results are compared to normal breast cells. Five different imaging channels are simultaneously examined within the emission wavelength range of 451-644 nm. Our results indicate that under near-infrared excitation, superior contrast of SK-BR-3 cancer cells labeled with immunotargeted nanoshells occurs at an emission wavelength ranging from 590 to 644 nm. Luminescence from labeled normal breast cells and autofluorescence from unlabeled cancer and normal cells remain imperceptible under the same conditions.
我们展示了使用免疫靶向金纳米壳作为体外双光子显微镜造影剂的能力。首先在780nm的近红外激发下验证了不同尺寸金纳米壳的双光子发光特性。然后探索了双光子显微镜作为一种工具对用抗HER2偶联纳米壳标记的活的HER2过表达乳腺癌细胞进行成像的效用，并将成像结果与正常乳腺细胞进行比较。在451-644nm的发射波长范围内同时检查了五个不同的成像通道。我们的结果表明，在近红外激发下，用免疫靶向纳米壳标记的SK-BR-3癌细胞在590至644nm的发射波长处具有优异的对比度。在相同条件下，标记的正常乳腺细胞的发光以及未标记的癌细胞和正常细胞的自发荧光仍然不可察觉。
