Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore117543.
Nanoscale. 2011 Feb;3(2):429-34. doi: 10.1039/c0nr00458h. Epub 2010 Oct 13.
The one- and two-photon excitation emission properties of water soluble glutathione monolayer protected gold clusters were investigated. Strong two-photon emission was observed from the gold clusters. The two-photon absorption cross section of these gold clusters in water was deduced from the z-scan measurement to be 189 740 GM, which is much higher compared to organic fluorescent dyes and quantum dots. These gold clusters also showed high photo-stability. The MTT assay showed that these gold clusters have low toxicity even at high concentrations. We have successfully demonstrated their applications for both one and two-photon excitation live cell imaging. The exceptional properties of these gold clusters make them a promising alternative for one- and two-photon bio-imaging and other nonlinear optical applications.
研究了水溶性谷胱甘肽单层保护金纳米簇的单光子和双光子激发发光性质。从金纳米簇中观察到了强双光子发射。通过 Z 扫描测量从水中这些金纳米簇的双光子吸收截面推断为 189740GM，与有机荧光染料和量子点相比，这要高得多。这些金纳米簇还表现出高的光稳定性。MTT 测定表明，即使在高浓度下，这些金纳米簇的毒性也很低。我们已经成功地证明了它们在单光子和双光子激发活细胞成像中的应用。这些金纳米簇的优异性能使它们成为单光子和双光子生物成像和其他非线性光学应用的一种很有前途的替代方法。
