Wada M, Kohno K, Imamoto F, Kano Y
Laboratory of Molecular Genetics, Institute of Physical and Chemical Research, Ibaraki, Japan.
Gene. 1988 Oct 30;70(2):393-7. doi: 10.1016/0378-1119(88)90211-9.
The fertility plasmid F'gal was not stably maintained in a hupA-hupB double mutant of Escherichia coli. Moreover, mini-F plasmids pFZY1, pFTC1 and pFTC2 were unable to transform the double mutant, though these plasmids efficiently transformed cells harboring a hupA or hupB single mutation. The composite plasmid pFHS1, which consists of the f5 DNA fragment of F plasmid and the whole DNA of a pSC101 derivative that carries a temperature-sensitive mutation for DNA replication, was not stably maintained in the hup double mutant at 42 degrees C. These findings strongly suggest that HU protein is required for ori2-dependent replication of the F plasmid.
生育质粒F'gal在大肠杆菌的hupA - hupB双突变体中不能稳定维持。此外，微型F质粒pFZY1、pFTC1和pFTC2无法转化该双突变体，尽管这些质粒能高效转化携带hupA或hupB单突变的细胞。由F质粒的f5 DNA片段和携带DNA复制温度敏感突变的pSC101衍生物的完整DNA组成的复合质粒pFHS1，在42摄氏度下于hup双突变体中不能稳定维持。这些发现有力地表明，HU蛋白是F质粒ori2依赖性复制所必需的。
