Altermatt H J, Gebbers J O, Arnold W, Laissue J A
Institute of Pathology, University of Bern, Switzerland.
ORL J Otorhinolaryngol Relat Spec. 1990;52(2):113-20. doi: 10.1159/000276115.
A panel of monoclonal and polyclonal antibodies has been used to study the epithelium of the extraosseous part of the human endolymphatic sac (ES) by immunohistochemistry. The ES epithelium reacted with several epithelial markers such as Lu-5, different anticytokeratins, antiepithelial membrane antigen, and anticarcinoembryonic antigen. Unexpectedly, all epithelial cells also revealed a strong positive reaction for the mesenchymal marker vimentin and for S-100 protein. 'Neuroendocrine', a neurosecretory antigen, and neuron-specific enolase reactivity was detected in a few epithelial cells. The results support the assumption that the ES epithelium is metabolically active and capable of secretion and resorption. These findings are in keeping with results of functional experiments in animals. The demonstration of neurosecretory antigen and neuron-specific enolase in some cells indicate that the epithelium may also have paracrine functions.
一组单克隆抗体和多克隆抗体已被用于通过免疫组织化学研究人类内淋巴囊（ES）骨外部分的上皮组织。ES上皮与几种上皮标志物发生反应，如Lu-5、不同的抗细胞角蛋白、抗上皮膜抗原和抗癌胚抗原。出乎意料的是，所有上皮细胞对间充质标志物波形蛋白和S-100蛋白也呈现出强阳性反应。在少数上皮细胞中检测到“神经内分泌”这种神经分泌抗原以及神经元特异性烯醇化酶反应性。这些结果支持了ES上皮具有代谢活性且能够进行分泌和重吸收的假设。这些发现与动物功能实验的结果一致。在一些细胞中神经分泌抗原和神经元特异性烯醇化酶的证实表明上皮可能也具有旁分泌功能。
