Takumida M, Ylikoski J, Pirvola U, Virtanen I
Department of Otolaryngology, Hiroshima University School of Medicine, Japan.
ORL J Otorhinolaryngol Relat Spec. 1991;53(1):10-4. doi: 10.1159/000276176.
The expression of the five main groups of intermediate filaments and their subgroups, especially cytokeratins, was investigated in the guinea pig endolymphatic sac at the light microscopic level using immunohistochemistry. Immunostaining for cytokeratin (PKK1, PKK2, PKK3) was found in the epithelial cell layer of the sac. Vimentin was seen to stain epithelial cell layer as well as subepithelial tissue. These findings may indicate that the cytokeratins are closely related to the inner ear fluid transport. The coexpression of cytokeratin and vimentin seemed to indicate the dual function of absorption and secretion of the endolymphatic sac epithelial cell to regulate the inner ear fluid homeostasis.
利用免疫组织化学技术，在光镜水平研究了豚鼠内淋巴囊中间丝的五个主要组及其亚组，尤其是细胞角蛋白的表达。在囊的上皮细胞层发现了细胞角蛋白（PKK1、PKK2、PKK3）的免疫染色。波形蛋白在上皮细胞层以及上皮下组织中均有染色。这些发现可能表明细胞角蛋白与内耳液体运输密切相关。细胞角蛋白和波形蛋白的共表达似乎表明内淋巴囊上皮细胞具有吸收和分泌的双重功能，以调节内耳液体稳态。
