Academia de Genómica, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, IPN Unidad Dgo., Durango, Zip Code 34220, Mexico.
Eur J Obstet Gynecol Reprod Biol. 2011 Nov;159(1):67-71. doi: 10.1016/j.ejogrb.2011.07.019. Epub 2011 Aug 9.
The aim of the present work was to look at differences in the placental tissue expression of KiSS-1 and REN genes from preeclamptic and healthy pregnant women, that could account for a possible synergistic function for both genes in the pathogenesis of preeclampsia.
This case-control study involved 27 preeclamptic women and 27 normoevolutive pregnant women. cDNA was obtained from placental tissue to carry out qPCR for both KiSS-1 and REN genes in order to compare mRNA expression levels in the studied groups. Statistical analysis showed expression differences that correlate with clinical and/or biochemical variables.
Higher expression for KiSS-1 in PEE vs. control woman (p=0.001) was observed, whereas no difference was observed for REN expression (p=0.300) when all the subjects were included. However, REN expression was significant higher when the samples were stratified according to preeclampsia severity. For 18 mild preeclamptic patients the p-value was p=0.001 compared to their controls, while for the remaining nine with severe preeclampsia the expression became significant (p=0.001).
Our results suggest that the high KiSS-1 expression seen in preeclamptic patients is in accordance with its role as an inhibitor of trophoblast invasiveness and maintained until the end of gestation. On the other hand, aggressive therapeutic management and/or severity status of patients have a direct effect on placental REN expression levels, masking the natural high expression of this gene on preeclamptic placental tissue. Therefore it was not possible to establish a real concordant expression profile for KiSS-1 and REN genes.
本研究旨在探讨子痫前期患者与健康孕妇胎盘组织中 Kiss-1 和 Ren 基因的表达差异，这些差异可能与这两种基因在子痫前期发病机制中的协同作用有关。
本病例对照研究纳入了 27 例子痫前期患者和 27 例正常妊娠孕妇。从胎盘组织中提取 cDNA，进行 Kiss-1 和 Ren 基因的 qPCR，以比较两组的 mRNA 表达水平。统计分析显示，表达差异与临床和/或生化变量相关。
与对照组相比，子痫前期患者的 Kiss-1 表达明显升高（p=0.001），而 Ren 基因的表达无差异（p=0.300）。然而，当根据子痫前期严重程度对样本进行分层时，Ren 基因的表达显著升高。对于 18 例轻度子痫前期患者，与对照组相比，p 值为 p=0.001，而对于其余 9 例重度子痫前期患者，表达变得显著（p=0.001）。
我们的研究结果表明，子痫前期患者中高表达的 Kiss-1 与其作为滋养细胞侵袭抑制剂的作用一致，并持续到妊娠晚期。另一方面，积极的治疗管理和/或患者的严重程度直接影响胎盘 Ren 基因的表达水平，掩盖了子痫前期胎盘组织中该基因的高自然表达。因此，未能建立 Kiss-1 和 Ren 基因的一致表达谱。
