School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
J Pharm Biomed Anal. 2011 Dec 5;56(4):815-9. doi: 10.1016/j.jpba.2011.07.015. Epub 2011 Jul 22.
In this study, a high performance liquid chromatography (HPLC) coupled with diode array detection (DAD) for simultaneous determination of six flavones including baicalein, sophoricoside, rutin, baicalin, quercetin and genistein in rat plasma and tissues after oral administration of JiangYaBifeng (JYBF) tablets was developed. The investigated analytes in plasma and tissues were extracted and purified with liquid-liquid extraction and solid phase extraction (SPE). Chromatographic separation was accomplished on a DIONEX Acclaim C18 column (250mm×4.6mm, 5.0μm particle size) with a simple linear gradient elution. The calibration curves for all the flavones had good linearity in the measured range with R(2) higher than 0.9983. The relative errors (REs) of the intra- and inter-day accuracy at different flavones levels were all less than ±10%. The proposed method enables unambiguous identification and quantification of investigated flavones in vivo. This is the first report on determination of the major flavones in rat plasma and tissues after oral administration of JYBF tablets. The results provided a meaningful basis for evaluating the clinical application of this medicine.
本研究建立了一种高效液相色谱（HPLC）-二极管阵列检测（DAD）法，用于同时测定大鼠血浆和组织中 6 种黄酮类化合物，包括黄芩苷、槐糖苷、芦丁、黄芩素、槲皮素和染料木苷，这些黄酮类化合物是口服姜雅避风（JYBF）片后的代谢产物。采用液液萃取和固相萃取（SPE）对血浆和组织中的分析物进行提取和纯化。色谱分离在 Dionex Acclaim C18 柱（250mm×4.6mm，5.0μm 粒径）上进行，采用简单的线性梯度洗脱。所有黄酮类化合物的校准曲线在测量范围内均具有良好的线性，相关系数（R2）均大于 0.9983。不同黄酮类化合物水平的日内和日间准确度的相对误差（RE）均小于±10%。该方法能够在体内明确鉴定和定量测定所研究的黄酮类化合物。这是首次报道口服 JYBF 片后大鼠血浆和组织中主要黄酮类化合物的测定。研究结果为评估该药物的临床应用提供了有意义的依据。
