Department of Chemistry, Zhengzhou University, Zhengzhou 450001, PR China.
J Pharm Biomed Anal. 2011 Jun 1;55(3):552-6. doi: 10.1016/j.jpba.2011.01.021. Epub 2011 Jan 22.
An effective, accurate and reliable method was developed for the simultaneous separation and determination of eight active components (baicalin, baicalein, sophoricoside, rutin, quercetin, genistein, pargyline and hydrochlorothiazide) in Chinese medicine 'JiangYaBiFeng' tablet (JYBF tablet) by high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD). Due to the different UV characteristic of these components, different wavelengths were selected for analysis of different analytes, such as 210nm for pargyline, 256nm for sophoricoside, rutin, quercetin and genistein, and 280nm for baicalin, baicalein and hydrochlorothiazide. Excellent linear behaviors over the investigated concentration ranges were observed with the values of R(2) higher than 0.9990 for all analytes. The recovery rates and relative standard deviation (RSD) for all analytes at three different concentrations were 94.9-104.7% and 1.23-3.00%, respectively. The validated method was successfully applied to the simultaneously determination of these active components in 'JiangYaBiFeng' tablet from different production batches, indicating that the proposed method in this paper was particularly suitable for the routine analysis of JYBF tablet and its quality control.
建立了一种高效、准确、可靠的高效液相色谱-二极管阵列检测法同时分离和测定中药降压避风片中 8 种活性成分（黄芩苷、黄芩素、槐糖苷、芦丁、槲皮素、染料木黄酮、帕吉林和氢氯噻嗪）的方法。由于这些成分的紫外特征不同，选择了不同的波长来分析不同的分析物，如 210nm 用于帕吉林，256nm 用于槐糖苷、芦丁、槲皮素和染料木黄酮，280nm 用于黄芩苷、黄芩素和氢氯噻嗪。所有分析物在考察的浓度范围内均表现出优异的线性行为，相关系数（R2）均高于 0.9990。所有分析物在三个不同浓度下的回收率和相对标准偏差（RSD）分别为 94.9-104.7%和 1.23-3.00%。该验证方法成功地应用于不同批次降压避风片中这些活性成分的同时测定，表明本文提出的方法特别适用于降压避风片的常规分析及其质量控制。
