Nano-Bio Field, Materials Nanoarchitectonics (MANA), National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
Colloids Surf B Biointerfaces. 2011 Nov 1;88(1):260-4. doi: 10.1016/j.colsurfb.2011.06.041. Epub 2011 Jul 23.
The effect of ultraviolet ray (UV) irradiation on the bonding strength between low carbon stainless steel 316 (SUS316L) and trisuccinimidyl citrate (TSC)-crosslinked alkali-treated gelatin (AlGelatin-TSC) was investigated. The UV irradiation effectively generated hydroxyl groups on the surface of SUS316L. The bonding strength between AlGelatin-TSC and SUS316L before UV irradiation was 0.345±0.007 MPa, and upon UV irradiation it increased to 0.750±0.069 MPa. In order to explain this enhanced bonding strength, the surface of SUS316L was examined using its water contact angle and X-ray photoelectron spectroscopy. Furthermore, the N 1s peaks derived from the TSC succinimidyl group were assigned to the surface of SUS316L after the immobilization of the TSC. This indicates that ester bond formation between the TSC active esters and the SUS316L hydroxyl groups contributed to the enhanced bonding strength. Therefore, UV irradiation and subsequent TSC immobilization is a simple way to functionalize biometal surfaces with various structures. This has practical applications for medical devices such as drug-eluting stents, dental implants, and metallic artificial bone.
研究了紫外线（UV）照射对低碳不锈钢 316（SUS316L）与三琥珀酰亚胺基丙基碳化二亚胺交联的碱处理明胶（AlGelatin-TSC）之间粘结强度的影响。UV 照射可在 SUS316L 表面有效生成羟基。在未进行 UV 照射时，AlGelatin-TSC 与 SUS316L 之间的粘结强度为 0.345±0.007 MPa，而经 UV 照射后，其粘结强度增加至 0.750±0.069 MPa。为了解释这种增强的粘结强度，使用 SUS316L 的水接触角和 X 射线光电子能谱对其表面进行了检查。此外，固定 TSC 后，源自 TSC 琥珀酰亚胺基的 N 1s 峰被分配到 SUS316L 的表面。这表明 TSC 活性酯与 SUS316L 羟基之间形成了酯键，从而增强了粘结强度。因此，UV 照射和随后的 TSC 固定是对具有各种结构的生物金属表面进行功能化的简单方法。这在药物洗脱支架、牙科植入物和金属人工骨等医疗器械方面具有实际应用。
