Sasaki Makoto, Inoue Motoki, Katada Yasuyuki, Nishida Yuuki, Taniguchi Akiyoshi, Hiromoto Sachiko, Taguchi Tetsushi
Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan; Biomaterials Unit, Nano-Bio Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Biomaterials Unit, Nano-Bio Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
Sci Technol Adv Mater. 2012 Dec 13;13(6):064213. doi: 10.1088/1468-6996/13/6/064213. eCollection 2012 Dec.
Calcium phosphate was formed on nickel-free high-nitrogen stainless steel (HNS) by chemical solution deposition. The calcium phosphate deposition was enhanced by glutamic acid covalently immobilized on the surface of HNS with trisuccinimidyl citrate as a linker. X-ray diffraction patterns and Fourier transform infrared spectra showed that the material deposited on glutamic acid-immobilized HNS within 24 h was low-crystallinity calcium-deficient carbonate-containing hydroxyapatite (HAp). The biological activity of the resulting HAp-coated HNS was investigated by using a human osteoblast-like MG-63 cell culture. The HAp-coated HNS stimulated the alkaline-phosphate activity of the MG-63 culture after 7 days. Therefore, HAp-coated HNS is suitable for orthopedic devices and soft tissue adhesion materials.
通过化学溶液沉积法在无镍高氮不锈钢（HNS）上形成了磷酸钙。以柠檬酸三琥珀酰亚胺酯为连接剂，将谷氨酸共价固定在HNS表面，增强了磷酸钙的沉积。X射线衍射图谱和傅里叶变换红外光谱表明，在24小时内沉积在固定有谷氨酸的HNS上的物质是低结晶度的含碳酸根的缺钙羟基磷灰石（HAp）。通过人成骨样MG-63细胞培养研究了所得HAp涂层HNS的生物活性。7天后，HAp涂层HNS刺激了MG-63培养物的碱性磷酸酶活性。因此，HAp涂层HNS适用于骨科器械和软组织粘附材料。
