Graduate School of Pure and Applied Science, University of Tsukuba, Tsukuba, Ibaraki, Japan.
J Mater Sci Mater Med. 2013 Apr;24(4):951-8. doi: 10.1007/s10856-012-4845-6. Epub 2013 Jan 19.
The surface of nickel-free high-nitrogen stainless steel (HNS) was modified with a citric acid-based cross-linker, trisuccinimidyl citrate (TSC), to promote initial cell adhesion in external skeletal fixation pins. The remaining active ester groups on TSC-immobilized HNS reacted with the amino groups of serum proteins. The immobilized serum proteins formed cell recognition sites to promote the initial cell adhesion immediately after cell seeding. The amount of fibronectin, which is a typical cell adhesion protein, immobilized on the TSC-immobilized HNS surface was threefold greater than on the original HNS after only 15 min. The fibroblastic cell culture experiments showed that the initial cell adhesion was significantly enhanced on the TSC-immobilized HNS compared with the original HNS at 3 h. Furthermore, the cell adhesion activity of the TSC-immobilized HNS continued to promote cell proliferation even at 7 days. Therefore, TSC-immobilized HNS may enable the rapid integration of soft tissues through its reaction with the patient's serum proteins and extracellular proteins around the surgical site.
无镍高氮不锈钢（HNS）的表面用柠檬酸基交联剂三（琥珀酰亚胺基）柠檬酸酯（TSC）进行了改性，以促进外骨骼固定钉中的初始细胞黏附。TSC 固定在 HNS 上的剩余活性酯基团与血清蛋白的氨基反应。固定的血清蛋白形成细胞识别位点，以促进细胞接种后立即进行初始细胞黏附。仅 15 分钟后，固定在 TSC 上的 HNS 表面上的纤连蛋白（一种典型的细胞黏附蛋白）的含量比原始 HNS 增加了两倍。成纤维细胞培养实验表明，与原始 HNS 相比，TSC 固定的 HNS 在 3 小时时初始细胞黏附显著增强。此外，即使在 7 天，TSC 固定的 HNS 的细胞黏附活性仍继续促进细胞增殖。因此，TSC 固定的 HNS 可能通过与患者的血清蛋白和手术部位周围的细胞外蛋白反应，实现软组织的快速整合。
