Department of Biosciences, TNO Quality of Life, Zeist, the Netherlands.
Benef Microbes. 2010 Mar;1(1):31-6. doi: 10.3920/BM2009.0009.
We have investigated the survival and activity of GanedenBC(30) during passage through the upper gastro-intestinal tract. GanedenBC(30) was tested in a dynamic, validated, in vitro model of the stomach and small intestine (TIM-1) on survival and its potential to aid in digestion of milk protein, lactose and fructose. The survival of GanedenBC(30) was high (70%), although germination of the spores was minimal (<10%) under the conditions tested. Survival of the strain in the presence of lactose and fructose was markedly lower (56-59%) than in the absence of the sugars. The amount of digested milk protein available for absorption was somewhat higher (+0.2 g) when GanedenBC(30) was added to the milk. When GanedenBC(30) was tested with lactose or fructose added to the meal, the cumulative amount of lactate produced was slightly higher (+0.12-0.18 mmol) compared to the GanedenBC(30) alone. In conclusion, although the differences in survival of GanedenBC(30) are small, these results show the potential of GanedenBC(30) to aid in protein digestion and in the digestion of lactose and fructose. If a larger fraction of the Bacillus coagulans cells had germinated, the influence on protein and carbohydrate digestion would probably have been much greater. Importance of the findings: the potential of GanedenBC(30) to aid in the digestion of lactose and fructose could be used to prevent occurrence of intestinal symptoms in individuals sensitive to these carbohydrates.
我们研究了 GanedenBC(30) 在通过上消化道时的存活和活性。GanedenBC(30) 在胃和小肠的动态、经过验证的体外模型 (TIM-1) 中进行了测试，以研究其在消化牛奶蛋白、乳糖和果糖方面的生存能力和潜在作用。GanedenBC(30) 的存活率很高 (70%)，尽管在测试条件下孢子的萌发率很低 (<10%)。在存在乳糖和果糖的情况下，该菌株的存活率明显低于 (56-59%) 没有糖的情况下。当添加 GanedenBC(30) 到牛奶中时，可用于吸收的消化牛奶蛋白的量略高 (+0.2 g)。当在含有乳糖或果糖的膳食中测试 GanedenBC(30) 时，与单独使用 GanedenBC(30) 相比，产生的乳酸累积量略高 (+0.12-0.18 mmol)。总之，尽管 GanedenBC(30) 的存活率差异很小，但这些结果表明 GanedenBC(30) 具有帮助消化蛋白质以及消化乳糖和果糖的潜力。如果凝结芽孢杆菌细胞的更大比例发芽，对蛋白质和碳水化合物消化的影响可能会更大。研究结果的重要性：GanedenBC(30) 有助于消化乳糖和果糖的潜力可用于预防对这些碳水化合物敏感的个体出现肠道症状。
