Microbiology and Fermentation Laboratory, Calpis Co. Ltd, 11-10, 5-Chome, Fuchinobe, Chuo-ku, 252-0206 Sagamiharashi, Kanagawa, Japan.
Benef Microbes. 2012 Sep;3(3):229-36. doi: 10.3920/BM2012.0016.
Survival and germination rate of Bacillus subtilis C-3102 spores were investigated in a stomach and small intestine model (TIM-1), while the impact of C-3102 cells that had passed through TIM-1 on human colon microbiota was evaluated in a model of the large intestine (TIM-2). The survival of C-3102 spores in TIM-1 was 99%; 8% of the spores had germinated. Effluent of TIM-1 was subsequently introduced into TIM-2 and a micro-array platform was employed to assess changes in the microbiota composition. The effluent, which contained germinated C-3102 cells, increased some Bifidobacterium species and decreased some Clostridium groups. These changes were greater compared to those obtained by adding C-3102 spores directly to TIM-2. The present study suggests that oral doses of B. subtilis C-3102 spores have the potential to modulate the human colon microbiota. This effect may be caused by germination of the spores in the gastrointestinal tract.
本研究采用胃-小肠模型（TIM-1）检测枯草芽孢杆菌 C-3102 孢子的存活和发芽率，采用大肠模型（TIM-2）评估穿过 TIM-1 的 C-3102 细胞对人结肠微生物群的影响。结果显示，C-3102 孢子在 TIM-1 中的存活率为 99%，有 8%的孢子发芽。随后将 TIM-1 的流出物引入 TIM-2，并采用微阵列平台评估微生物群组成的变化。含有发芽的 C-3102 细胞的流出物增加了一些双歧杆菌属物种，减少了一些梭菌属群体。与直接将 C-3102 孢子添加到 TIM-2 中相比，这些变化更为显著。本研究表明，口服枯草芽孢杆菌 C-3102 孢子有可能调节人体结肠微生物群。这种作用可能是由于孢子在胃肠道中发芽所致。
