Marteau P, Minekus M, Havenaar R, Huis in't Veld J H
Hôpital Saint-Lazare INSERM U290, Paris, France.
J Dairy Sci. 1997 Jun;80(6):1031-7. doi: 10.3168/jds.S0022-0302(97)76027-2.
This study was conducted to validate a dynamic model of the stomach and small intestine to quantify the survival of lactic acid bacteria and to assess the influence of gastrointestinal secretions. The survival of a single strain of each of the following species, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus, was measured under physiological conditions (e.g., peristalsis, changes in pH, and changes in concentrations of enzymes and bile) and were compared with data obtained from humans. No significant differences were found between the in vitro and in vivo data, indicating that the model has a predictive value for the survival of these bacteria in humans. The survival of these strains of lactic acid bacteria in the gastrointestinal model was investigated under two different conditions in the small intestine: simulation of physiological secretion of bile and low bile secretion. Reductions in viability were significantly different between the bacterial species. The dose-response effect of bile on the survival of the tested bacteria was significant, demonstrating the bactericidal effect of bile salts. This study demonstrates the differences among bacterial species in their sensitivity to gastric and intestinal secretions.
本研究旨在验证胃和小肠的动态模型，以量化乳酸菌的存活率，并评估胃肠分泌物的影响。在生理条件下（如蠕动、pH值变化、酶和胆汁浓度变化）测量了以下每种双歧双歧杆菌、嗜酸乳杆菌、保加利亚乳杆菌和嗜热链球菌单一菌株的存活率，并与从人类获得的数据进行了比较。体外和体内数据之间未发现显著差异，表明该模型对这些细菌在人体内的存活率具有预测价值。在小肠的两种不同条件下研究了这些乳酸菌菌株在胃肠模型中的存活率：模拟胆汁的生理分泌和低胆汁分泌。不同细菌种类的活力降低存在显著差异。胆汁对受试细菌存活率的剂量反应效应显著，证明了胆盐的杀菌作用。本研究证明了不同细菌种类对胃和肠分泌物的敏感性存在差异。
