Estonian University of Life Sciences, Institute Agricultural and Environmental Sciences, Department of Plant Protection, Kreutzwaldi 1, Tartu 51014, Estonia.
J Exp Biol. 2011 Sep 1;214(Pt 17):2816-22. doi: 10.1242/jeb.056051.
Oxygen convective uptakes in gas exchange cycles were directly recorded in early diapause pupae of Pieris brassicae L. (Lepidoptera; Pieridae) by means of O2 coulometric respirometry. This method was combined with flow-through CO2 respirometry, the two systems being switchable one to the other. During recording with both systems, measurements were also taken with infrared actography. The pupae displayed short discontinuous gas exchange cycles lasting 40-70 min. No true C phase was found by flow-through measurements; instead, flutter opening of the spiracles with discrete convective O2 uptakes began shortly after the O phase whereas CO2 release was suppressed by the inward directed passive suction ventilation. The F phase was characterized by a series of small CO2 bursts (flutter events). Between these bursts, novel sub-phase `miniflutter' was observed, which consisted of six to 10 miniature inspirations without any CO2 emission. During the flow-through measurements, oxygen convective uptakes were indirectly recorded by the infrared actograph as sudden extensions (lengthening) of the abdominal segments at each spiracular microopening.
利用 O2 库仑呼吸测定法，直接记录了小菜粉蝶（鳞翅目；粉蝶科）早期滞育蛹的气体交换循环中的氧气对流摄取量。该方法与流动 CO2 呼吸测定法相结合，两个系统可以相互切换。在使用这两个系统进行记录时，还使用红外动作记录仪进行了测量。蛹表现出短暂的不连续气体交换循环，持续时间为 40-70 分钟。通过流动测量没有发现真正的 C 相；相反，在 O 相之后不久，呼吸孔的颤动打开伴随着离散的对流氧气摄取开始，而 CO2 的释放则被内向的被动抽吸通风所抑制。F 相的特征是一系列小的 CO2 爆发（颤动事件）。在这些爆发之间，观察到了新的亚相miniflutter，它由六个到十个微型吸气组成，没有任何 CO2 排放。在流动测量期间，氧气对流摄取量通过红外动作记录仪间接记录，表现为每次呼吸孔微开启时腹部节段的突然伸展（延长）。
