Struckmeier Jens, Wahl Reiner, Leuschner Mirko, Nunes Joao, Janovjak Harald, Geisler Ulrich, Hofmann Gerd, Jähnke Torsten, Müller Daniel J
Cellular Machines, Biotechnology Center, Technische Universität Dresden, Tatzberg 47, D-01307 Dresden, Germany. nAmbition GmbH, Tatzberg 47, D-01307 Dresden, Germany.
Nanotechnology. 2008 Sep 24;19(38):384020. doi: 10.1088/0957-4484/19/38/384020. Epub 2008 Aug 12.
With the introduction of single-molecule force spectroscopy (SMFS) it has become possible to directly access the interactions of various molecular systems. A bottleneck in conventional SMFS is collecting the large amount of data required for statistically meaningful analysis. Currently, atomic force microscopy (AFM)-based SMFS requires the user to tediously 'fish' for single molecules. In addition, most experimental and environmental conditions must be manually adjusted. Here, we developed a fully automated single-molecule force spectroscope. The instrument is able to perform SMFS while monitoring and regulating experimental conditions such as buffer composition and temperature. Cantilever alignment and calibration can also be automatically performed during experiments. This, combined with in-line data analysis, enables the instrument, once set up, to perform complete SMFS experiments autonomously.
随着单分子力谱（SMFS）的引入，直接研究各种分子系统间的相互作用成为可能。传统单分子力谱的一个瓶颈在于收集具有统计学意义的分析所需的大量数据。目前，基于原子力显微镜（AFM）的单分子力谱需要用户繁琐地“钓取”单个分子。此外，大多数实验和环境条件必须手动调节。在此，我们开发了一种全自动单分子力谱仪。该仪器能够在监测和调节诸如缓冲液成分和温度等实验条件的同时进行单分子力谱分析。在实验过程中还能自动进行悬臂梁校准和对齐。这与在线数据分析相结合，使得该仪器一旦设置好，就能自主完成完整的单分子力谱实验。
