An Chenyi, Chen Wei
Department of Cell Biology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310058, China.
Key Laboratory for Biomedical Engineering of Ministry of Education and State Key Laboratory for Modern Optical Instrumentation, Zhejiang University, Hangzhou 310058, China.
Biophys Rep. 2021 Oct 31;7(5):377-383. doi: 10.52601/bpr.2021.210022.
Complex physical cues including two-dimensional membrane environment, dynamic mechanical force, and bioelectric activity inevitably affect membrane receptor functions. Multiplexed single-molecule force spectroscopy (SMFS) techniques with the capability of live-cell measurements are essential to systemically dissect receptor's functions under complex biophysical regulation. In this review, we summarize recent progress of live-cell based SMFS techniques and specifically focus on the progress of SMFS on the biomembrane force probe with enhanced mechanical stability and multiplexed capability of fluorescence imaging. We further suggest the necessity of developing multiplexed SMFS techniques with simultaneous bioelectric regulation capability to investigate membrane potential regulated membrane receptor functions. These state-of-art multiplexed SMFS techniques will dissect membrane receptors functions in a systematic biophysical angle, resolving the biochemical, biomechanical and bioelectrical regulatory mechanisms in physiologically relevant conditions.
包括二维膜环境、动态机械力和生物电活动在内的复杂物理线索不可避免地会影响膜受体功能。具有活细胞测量能力的多重单分子力谱（SMFS）技术对于系统剖析复杂生物物理调节下受体的功能至关重要。在本综述中，我们总结了基于活细胞的SMFS技术的最新进展，并特别关注具有增强机械稳定性和荧光成像多重能力的生物膜力探针上的SMFS进展。我们进一步提出，开发具有同步生物电调节能力的多重SMFS技术对于研究膜电位调节的膜受体功能是必要的。这些先进的多重SMFS技术将从系统的生物物理角度剖析膜受体功能，解析生理相关条件下的生化、生物力学和生物电调节机制。
