Wang Yanyan, Wang Yusong, Liu Bin
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117567, Singapore.
Nanotechnology. 2008 Oct 15;19(41):415605. doi: 10.1088/0957-4484/19/41/415605. Epub 2008 Sep 4.
Novel methods for rapid, sensitive and low-cost biomolecule detection have attracted particular interest because of their wide use in medical diagnostics, food inspection and biomedical research applications. In this work, we report a simple and efficient silica nanoparticle (NP)-based fluorescent assay for ATP detection. It takes advantage of the washing and separation properties of NPs and the structure-switch property of DNA aptamers, resulting in fluorescence change of the supernatant in the presence of targets. A linear response for ATP detection was observed from 0 to 6 mM with a detection limit of ∼34 µM. This detection strategy could be generalized to other aptamer-based detection systems.
新型的快速、灵敏且低成本的生物分子检测方法因其在医学诊断、食品检测和生物医学研究应用中的广泛使用而备受关注。在这项工作中，我们报道了一种基于二氧化硅纳米颗粒（NP）的简单高效的荧光法用于ATP检测。它利用了纳米颗粒的洗涤和分离特性以及DNA适配体的结构转换特性，在有靶标的情况下导致上清液的荧光发生变化。在0至6 mM范围内观察到ATP检测的线性响应，检测限约为34 μM。这种检测策略可以推广到其他基于适配体的检测系统。
