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基于在微流控反应器中合成的经适配体修饰的 PDA@POSS 纳米粒子的用于检测 ATP 的生物传感器。

Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor.

机构信息

Dept. Materials Sci. & Eng., A.T. Adana Sci. & Tech. Uni., Adana, 01250, Turkey.

Microfluidics & Lab-on-a-chip Research Group, İ.D. Bilkent Uni., Ankara, 06800, Turkey.

出版信息

Mikrochim Acta. 2024 Feb 23;191(3):153. doi: 10.1007/s00604-024-06186-7.

DOI:10.1007/s00604-024-06186-7
PMID:38393379
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10891265/
Abstract

This study introduces aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for adenosine triphosphate (ATP) detection where the POSS nanoparticles were synthesized in a one-step, continuous flow microfluidic reactor utilizing thermal polymerization. A microemulsion containing POSS monomers was generated in the microfluidic reactor which was designed to prevent clogging by using a continuous oil flow around the emulsion during thermal polymerization. Surfaces of POSS nanoparticles were biomimetically modified by polydopamine. The aptamer sequence for ATP was successfully attached to POSS nanoparticles. The aptamer-modified POSS nanoparticles were tested for affinity-based biosensor applications using ATP as a model molecule. The nanoparticles were able to capture ATP molecules successfully with an affinity constant of 46.5 [Formula: see text]M. Based on this result, it was shown, for the first time, that microfluidic synthesis of POSS nanoparticles can be utilized in designing aptamer-functionalized nanosystems for biosensor applications. The integration of POSS in biosensing technologies not only exemplifies the versatility and efficacy of these nanoparticles but also marks a significant contribution to the field of biorecognition and sample preparation.

摘要

本研究介绍了适体功能化多面体低聚倍半硅氧烷（POSS）纳米粒子用于三磷酸腺苷（ATP）检测，其中 POSS 纳米粒子是在一步法、连续流动微流控反应器中利用热聚合合成的。在微流控反应器中生成了包含 POSS 单体的微乳液，该反应器的设计利用了在热聚合过程中连续的油流围绕乳液，以防止堵塞。POSS 纳米粒子的表面通过聚多巴胺进行仿生修饰。适体序列成功地连接到 POSS 纳米粒子上。使用 ATP 作为模型分子，对适体修饰的 POSS 纳米粒子进行了基于亲和力的生物传感器应用测试。这些纳米粒子能够成功地捕获 ATP 分子，亲和力常数为 46.5 [Formula: see text]M。基于这一结果，首次表明 POSS 纳米粒子的微流合成可用于设计适体功能化纳米系统用于生物传感器应用。将 POSS 整合到生物传感技术中不仅例证了这些纳米粒子的多功能性和有效性，而且对生物识别和样品制备领域做出了重要贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/bc3c319cdd52/604_2024_6186_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/8ac9e5ca0c5f/604_2024_6186_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/38ec3426014a/604_2024_6186_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/c6df25f6a484/604_2024_6186_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/6c6f8b6f5c12/604_2024_6186_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/bc3c319cdd52/604_2024_6186_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/8ac9e5ca0c5f/604_2024_6186_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/38ec3426014a/604_2024_6186_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/c6df25f6a484/604_2024_6186_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/6c6f8b6f5c12/604_2024_6186_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8eb7/10891265/bc3c319cdd52/604_2024_6186_Fig5_HTML.jpg

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