Sharma Anjney, Singh Rajesh Kumar, Singh Pratiksha, Vaishnav Anukool, Guo Dao-Jun, Verma Krishan K, Li Dong-Ping, Song Xiu-Peng, Malviya Mukesh Kumar, Khan Naeem, Lakshmanan Prakash, Li Yang-Rui
Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Guangxi Key Laboratory of Crop Genetic Improvement and Biotechnology, Nanning 530007, China.
Microorganisms. 2021 Oct 22;9(11):2203. doi: 10.3390/microorganisms9112203.
Soil salinity causes severe environmental stress that affects agriculture production and food security throughout the world. Salt-tolerant plant-growth-promoting rhizobacteria (PGPR) and nitric oxide (NO), a distinctive signaling molecule, can synergistically assist in the alleviation of abiotic stresses and plant growth promotion, but the mechanism by which this happens is still not well known. In the present study, in a potential salt-tolerant rhizobacteria strain, ASN-1, growth up to 15% NaCl concentration was achieved with sugarcane rhizosphere soil. Based on 16S-rRNA gene sequencing analysis, the strain ASN-1 was identified as a . Strain ASN-1 exhibits multiple plant-growth-promoting attributes, such as the production of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate deaminase, siderophores, HCN, ammonia, and exopolysaccharides as well as solubilized phosphate solubilization. Biofilm formation showed that NO enhanced the biofilm and root colonization capacity of the PGPR strain ASN-1 with host plants, evidenced by scanning electron microscopy. The greenhouse study showed that, among the different treatments, the combined application of PGPR and sodium nitroprusside (SNP) as an NO donor significantly ( ≤ 0.05) enhanced sugarcane plant growth by maintaining the relative water content, electrolyte leakage, gas exchange parameters, osmolytes, and Na/K ratio. Furthermore, PGPR and SNP fertilization reduced the salinity-induced oxidative stress in plants by modulating the antioxidant enzyme activities and stress-related gene expression. Thus, it is believed that the acquisition of advanced information about the synergistic effect of salt-tolerant PGPR and NO fertilization will reduce the use of harmful chemicals and aid in eco-friendly sustainable agricultural production under salt stress conditions.
土壤盐渍化会造成严重的环境压力，影响全球的农业生产和粮食安全。耐盐促植物生长根际细菌（PGPR）和一氧化氮（NO）作为一种独特的信号分子，可以协同帮助缓解非生物胁迫并促进植物生长，但其作用机制仍不清楚。在本研究中，在一种潜在的耐盐根际细菌菌株ASN-1中，利用甘蔗根际土壤实现了在高达15%氯化钠浓度下的生长。基于16S-rRNA基因测序分析，菌株ASN-1被鉴定为一种……。菌株ASN-1表现出多种促植物生长特性，如吲哚-3-乙酸、1-氨基环丙烷-1-羧酸脱氨酶、铁载体、HCN、氨和胞外多糖的产生以及溶解磷的能力。生物膜形成表明，NO增强了PGPR菌株ASN-1与寄主植物的生物膜和根部定殖能力，扫描电子显微镜证明了这一点。温室研究表明，在不同处理中，PGPR与作为NO供体的硝普钠（SNP）联合应用通过维持相对含水量、电解质渗漏、气体交换参数、渗透物质和Na/K比，显著（P≤0.05）促进了甘蔗植株生长。此外，PGPR和SNP施肥通过调节抗氧化酶活性和与胁迫相关的基因表达，降低了盐胁迫诱导的植物氧化应激。因此，人们认为，获取有关耐盐PGPR和NO施肥协同效应的先进信息将减少有害化学物质的使用，并有助于在盐胁迫条件下实现生态友好型可持续农业生产。
