Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, Uttar Pradesh 201307, India.
Bull Environ Contam Toxicol. 2011 Nov;87(5):522-6. doi: 10.1007/s00128-011-0382-0. Epub 2011 Aug 12.
In this study, two bacterial isolates designated Alp6 and Alp7 were isolated from soil collected from dye industries and screened for their ability to degrade carbazole. Growing cells of the isolates Alp6 and Alp7 could degrade 99.9% and 98.5% of carbazole, respectively in 216 h. The specific activity for degradation by the resting cells of Alp6 was found to be 7.96 μmol/min/g dry cell weight, while for Alp7 it was 5.82 μmol/min/g dry cell weight. Phylogenetic analysis based on 16S rDNA gene sequences showed that isolates Alp6 and Alp7 belonged to the genus Acinetobacter. To the best of our knowledge, this is the first report on the Acinetobacter spp. showing utilization of carbazole as carbon and nitrogen source.
在这项研究中，从染料工业采集的土壤中分离出了两个细菌分离株，分别命名为 Alp6 和 Alp7，并筛选它们降解咔唑的能力。分离株 Alp6 和 Alp7 的生长细胞分别在 216 小时内降解了 99.9%和 98.5%的咔唑。发现 Alp6 静止细胞的降解比活性为 7.96 μmol/min/g 干细胞重量，而 Alp7 的比活性为 5.82 μmol/min/g 干细胞重量。基于 16S rDNA 基因序列的系统发育分析表明，分离株 Alp6 和 Alp7 属于不动杆菌属。据我们所知，这是首次报道不动杆菌属利用咔唑作为碳源和氮源。
