Zhang Xiao-Fan, He Yi-Liang
School of Environmental Science and Engineering Shanghai Jiaotong University, Shanghai 200240, China.
Huan Jing Ke Xue. 2010 Sep;31(9):2166-70.
A bacterial strain was isolated from soil samples using plate screening techniques. Results indicated this isolated were able to use carbazole as sole source of carbon and energy, simultaneously, including N-Methylcarbazole, 4-Hydroxycarbazole and 2,2'-Biphenol. It was identified as Flavobacterium sp. according to its morphology, and biochemical properties, and 16S rDNA sequence analysis. Utilization of carbazole by the isolates was confirmed by the increase in bacterial biomass and the decrease in substrate concentration in liquid cultures. The optimal pH and temperature for cell growth and carbazole degradation were 7.5 and 30 degrees C, respectively. Resting cells grown in Luria broth also showed activity for decomposing other heterocyclic compounds. In addition, biodegradation of carbazole was carried out with carbazole degrading strain KH-6. The results indicated that 90% of the carbazole could be degraded in the sterilized soil. And strain KH-6 could enhance the degradations of carbazole significantly.
采用平板筛选技术从土壤样品中分离出一株细菌菌株。结果表明，该分离菌株能够以咔唑作为唯一碳源和能源，同时包括N-甲基咔唑、4-羟基咔唑和2,2'-联苯酚。根据其形态、生化特性以及16S rDNA序列分析，将其鉴定为黄杆菌属。通过液体培养中细菌生物量的增加和底物浓度的降低，证实了该分离菌株对咔唑的利用。细胞生长和咔唑降解的最适pH值和温度分别为7.5和30℃。在Luria肉汤中生长的静止细胞也表现出分解其他杂环化合物的活性。此外，用咔唑降解菌株KH-6进行了咔唑的生物降解。结果表明，在灭菌土壤中90%的咔唑能够被降解。并且菌株KH-6能够显著增强咔唑的降解。
