Molecular Biotechnology Laboratory, Department of Biology, Faculty of Sciences, Shiraz University, P. O. Box 71467-13565, Shiraz, Iran.
World J Microbiol Biotechnol. 2012 Oct;28(10):3075-80. doi: 10.1007/s11274-012-1105-3. Epub 2012 Jun 20.
In this work, a bacterial strain with suitable capability to metabolize carbazole (CAR) as a main nitrogen containing compound of petroleum was isolated and characterized. 16S rDNA gene analysis and morphological characteristics of the strain showed that the isolate belonged to the genus Achromobacter and was tentatively named as Achromobacter sp. strain CAR1389. The growth monitoring and biodegradation rate measurements of carbazole in minimal medium supplemented by 6 mM CAR revealed that the strain CAR1389 is able to remove more than 90 % of this compound at 25, 30, and 37 °C during 7 days. The effect of higher concentrations of the carbazole on growth rate and metabolizing activity of the strain exhibited the Achromobacter sp. strain CAR1389 can tolerate increasing levels of CAR concentration up to 21 mM in culture media and degrade 43 % of this toxic material. According to these results and high tolerance of this bacterium in regards to higher concentrations of CAR, we suggest the strain CAR1389 as a suitable isolate to do biorefining of crude oil and also bioremediation processes in highly contaminated area of carbazole.
在这项工作中，分离并鉴定了一种能够代谢咔唑（CAR）作为石油主要含氮化合物的细菌菌株。16S rDNA 基因分析和菌株的形态特征表明，该分离物属于无色杆菌属，并暂定名为 Achromobacter sp. strain CAR1389。在补充有 6mM CAR 的最小培养基中监测咔唑的生长和生物降解率，结果表明，CAR1389 菌株在 25、30 和 37°C 下 7 天内能够去除超过 90%的这种化合物。较高浓度咔唑对菌株生长速率和代谢活性的影响表明，无色杆菌属菌株 CAR1389 能够耐受培养基中 CAR 浓度增加到 21mM，并降解 43%的这种有毒物质。根据这些结果以及该细菌对较高浓度 CAR 的高耐受性，我们建议将菌株 CAR1389 作为一种合适的分离物，用于原油的生物炼制以及咔唑污染严重地区的生物修复过程。
