Dos Santos Patricia C
Department of Chemistry, Wake Forest University, Wiston-Salem, NC 27109, USA.
Methods Mol Biol. 2011;766:81-92. doi: 10.1007/978-1-61779-194-9_6.
Biological nitrogen fixation is a complex and tightly regulated process limited to a group of prokaryotic species known as diazotrophs. Among well-studied diazotrophs, Azotobacter vinelandii is the best studied for its convenience of aerobic growth, its high levels of nitrogenase expression, and its genetic tractability. This chapter includes protocols and strategies in the molecular biology and genetic engineering of A. vinelandii that have been used as valuable tools for advancing studies on the biosynthesis, mechanism, and regulation of nitrogen fixation.
生物固氮是一个复杂且受到严格调控的过程，仅限于一类被称为固氮菌的原核生物。在经过充分研究的固氮菌中，维涅兰德固氮菌因其有氧生长的便利性、高水平的固氮酶表达以及遗传易处理性而得到了最为深入的研究。本章介绍了维涅兰德固氮菌分子生物学和基因工程方面的方案和策略，这些已被用作推进固氮生物合成、机制及调控研究的宝贵工具。
