Inserm, U, Paris, France.
Biol Blood Marrow Transplant. 2012 Jan;18(1):150-5. doi: 10.1016/j.bbmt.2011.08.002. Epub 2011 Aug 9.
Although rodent graft-versus-host disease (GVHD) models have suggested that indoleamine 2,3-dioxygenase (IDO) is a critical regulator of gastrointestinal GVHD, parallel human studies on IDO expression have not been reported. IDO expression was assessed in 20 patients who underwent duodenal biopsy. IDO was upregulated in epithelial cells. In situ analyses reveal that macrophages and dendritic cells stain positive for IDO, but that most of the IDO(+) cells were a novel population of CD3(+)CD4(+)IDO(+) cells. The proportion of CD4(+)IDO(+) T cells was significantly higher in patients with moderate GVHD. In situ regulatory T cell and Th17 numbers correlated with overall severity. Although needing confirmatory results from larger sample sets, these data are consistent with the hypothesis that IDO is involved in regulating gastrointestinal GVHD.
尽管啮齿动物移植物抗宿主病 (GVHD) 模型表明色氨酸 2,3-双加氧酶 (IDO) 是胃肠道 GVHD 的关键调节因子，但尚未有关于 IDO 表达的平行人类研究报告。对 20 例接受十二指肠活检的患者进行 IDO 表达评估。上皮细胞中 IDO 上调。原位分析显示巨噬细胞和树突状细胞呈 IDO 阳性，但大多数 IDO(+)细胞是一种新型的 CD3(+)CD4(+)IDO(+)细胞群。中度 GVHD 患者的 CD4(+)IDO(+)T 细胞比例明显更高。原位调节性 T 细胞和 Th17 细胞数量与总体严重程度相关。尽管需要更大样本量的确认结果，但这些数据与 IDO 参与调节胃肠道 GVHD 的假设一致。
