幽门螺杆菌感染胃黏膜中吲哚胺 2,3-双加氧酶表达增加调节 Th1/Th17/Th22 和 Treg 途径。

Increased Indoleamine 2, 3-Dioxygenase expression modulates Th1/Th17/Th22 and Treg pathway in humans with Helicobacter Pylori-Infected gastric mucosa.

机构信息

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

出版信息

Hum Immunol. 2021 Jan;82(1):46-53. doi: 10.1016/j.humimm.2020.10.005. Epub 2020 Oct 28.

DOI:10.1016/j.humimm.2020.10.005

PMID:33127161

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8414194/

Abstract

INTRODUCTION AND PURPOSE

Indoleamine 2, 3- dioxygenase (IDO) plays an importantrole in immunosuppressive pathway, as inhibits responsesof T cells and promotes immune tolerance. Host responsetoHelicobacter pylori (H. pylori) is involved in the infection persistenceand it is also associatedwith different clinical outcomes. The aim of this study was to investigate the role of IDO in H. pylori-infected patients with gastritis diseases and peptic ulcer diseases (PUD) through the assessment of the relationship among IDO protein expression and the numbers of T helper (Th)-1, Th17, Th22, and T regulator (Treg) cells.

MATERIALS AND METHODS

Antrum biopsy was obtained from H. pylori-negative patients (n = 48) and H. pylori-positive subjects (55 patients with gastritis and 47 patients with PUD), for performing H. pylori status and histopathological assessments. IDO protein expression was evaluated by Western blotting.

RESULTS

IDO protein expression was significantly higher in gastric biopsies from H. pylori-positive subjects compared to the H. pylori-negative subjects, and also in H. pylori-positive subjects with gastritis disease compared to H. pylori-positive subjects with PUD. Moreover, in H. pylori-positive subjects, a positive correlation was observed between IDO protein expression and the frequency of Treg cells. In addition, a negative correlation was observed between IDO protein expression and the number of Th1, Th17, and Th22.

CONCLUSION

Increased IDO protein expression is able to change the number of Th1, Th17, Th22, and Treg cells and these changes are possibly associated with an increase in the risk of PUD development in H. pylori-infected patients.

摘要

简介与目的

色氨酸 2,3-双加氧酶（IDO）在免疫抑制途径中发挥重要作用，可抑制 T 细胞的反应并促进免疫耐受。宿主对幽门螺杆菌（H. pylori）的反应与感染的持续存在有关，也与不同的临床结果有关。本研究旨在通过评估 IDO 蛋白表达与辅助性 T 细胞（Th）-1、Th17、Th22 和调节性 T 细胞（Treg）数量之间的关系，来研究 IDO 在 H. pylori 感染的胃炎和消化性溃疡病（PUD）患者中的作用。

材料与方法

从 H. pylori 阴性患者（n=48）和 H. pylori 阳性患者（55 例胃炎患者和 47 例 PUD 患者）中获取胃窦活检，进行 H. pylori 状态和组织病理学评估。通过 Western blot 评估 IDO 蛋白表达。

结果

与 H. pylori 阴性患者相比，H. pylori 阳性患者的胃活检组织中 IDO 蛋白表达显著升高，且在 H. pylori 阳性的胃炎患者中高于 H. pylori 阳性的 PUD 患者。此外，在 H. pylori 阳性患者中，IDO 蛋白表达与 Treg 细胞的频率呈正相关。另外，IDO 蛋白表达与 Th1、Th17 和 Th22 的数量呈负相关。