Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Gene. 2011 Nov 1;487(1):103-6. doi: 10.1016/j.gene.2011.07.019. Epub 2011 Aug 2.
Increased RANTES expression has been described to have a role in atherosclerosis plaque formation. Functional polymorphisms within RANTES promoter region have shown association with increased risk of coronary atherosclerosis (CAD). The aim of this study was to examine the RANTES mRNA expression in patients with CAD compared to patients without CAD and its association with RANTES -403 G/A polymorphism in an Iranian population.
The study was performed on 319 patients who underwent coronary artery angiography and patients with >50% stenosis in vessels considered as case groups (CAD+) N=191 and normal vessels group as control (CAD-) N=128. In each group 20 patients were examined for RANTES mRNA expression. RANTES mRNA expression was examined using quantitative real-time PCR. Genotyping of -403 polymorphism was performed using PCR-RFLP technique.
We found that RANTES mRNA expression was increased to 1.37 fold in CAD patients compared to the controls but the difference was not statistically significant. Also comparing the RANTES mRNA expression in patients with different RANTES -403 G/A polymorphism showed that in patients carrying AA genotype RANTES mRNA expression was increased to 1.74 fold compared to patients carrying GG genotype and to 1.51 fold compared to patients carrying GA genotype. No significant difference for allele and genotype frequencies of RANTES -403 polymorphism was found between cases and controls.
More studies on larger number of samples are required to further evaluate role of RANTES in pathogenesis of CAD.
RANTES 表达增加被认为在动脉粥样硬化斑块形成中起作用。RANTES 启动子区域的功能多态性与冠状动脉粥样硬化（CAD）风险增加相关。本研究旨在检测伊朗人群 CAD 患者与无 CAD 患者的 RANTES mRNA 表达，并与 RANTES-403G/A 多态性相关联。
对 319 例接受冠状动脉造影的患者进行了研究，将血管狭窄>50%的患者视为病例组（CAD+）N=191 例，正常血管组作为对照组（CAD-）N=128 例。在每组中，检查了 20 例患者的 RANTES mRNA 表达。使用实时定量 PCR 检测 RANTES mRNA 表达。使用 PCR-RFLP 技术检测 -403 多态性的基因分型。
我们发现 CAD 患者的 RANTES mRNA 表达增加了 1.37 倍，但与对照组相比差异无统计学意义。此外，比较 RANTES-403G/A 多态性不同患者的 RANTES mRNA 表达，发现携带 AA 基因型的患者 RANTES mRNA 表达增加了 1.74 倍，与携带 GG 基因型的患者相比，与携带 GA 基因型的患者相比增加了 1.51 倍。病例组和对照组之间 RANTES-403 多态性的等位基因和基因型频率无显著差异。
需要对更多的样本进行更多的研究，以进一步评估 RANTES 在 CAD 发病机制中的作用。
